CD39/ENTPD1 Rekombinanter Antikörper

CD39/ENTPD1 Rekombinant Antikörper für WB, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

250764B5

Kat-Nr. : 86327-1-PBS

Synonyme

ATP diphosphohydrolase, ATPDase, ATP-DPH, CD39, EC:3.6.1.5

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  WB analysis using 86327-1-RR (same clone as 86327-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86327-1-RR (CD39 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86327-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse lung using 86327-1-RR (same clone as 86327-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 86327-1-RR (CD39 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86327-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA standard curve of MP02378-2

  Sandwich ELISA standard curve of MP02378-2, Mouse CD39/ENTPD1 Recombinant Matched Antibody Pair - PBS only. 86327-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg3483. 86327-1-PBS was HRP conjugated as the detection antibody. Range: 78.1-5000 pg/mL.

• 

  Sample test of MP02378-2

  The mean CD39/ENTPD1 concentration was determined to be 926.3 ng/mL in mouse spleen extract based on a 8.5 mg/mL extract load and 2,212.6 ng/mL in mouse lung extract based on a 5.9 mg/mL extract load.

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Geprüfte Anwendungen

Produktinformation

86327-1-PBS bindet in WB, Sandwich ELISA, Indirect ELISA, Sample test CD39/ENTPD1 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD39/ENTPD1 fusion protein Eg3483
• Vollständiger Name: ectonucleoside triphosphate diphosphohydrolase 1
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: NM_009848.4
• Gene symbol: Entpd1
• Gene ID (NCBI): 12495
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ENTPD1 (Ectonucleoside triphosphate diphosphohydrolase 1) is also named CD39, NTPDase 1, and Ecto-ATPDase 1 and belongs to the GDA1/CD39 NTPase family. In the nervous system, it can hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. It is a noncovalent tetrameric protein and detergent inhibition of enzymatic activity is caused by dissociation of ectoapyrase tetramers into monomers(PMID: 9733785 ). And it is a 70- to 100-kDa protein that is heavily N-glycosylated (PMID:7930580). This protein has 6 glycosylation sites and 3 isoforms (58 kDa, 59 kDa, and 34 kDa) produced by alternative splicing.