CD39/ENTPD1 Rekombinanter Antikörper

CD39/ENTPD1 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

250764B5

Kat-Nr. : 86327-1-RR

Synonyme

ATP diphosphohydrolase, ATPDase, ATP-DPH, CD39, EC:3.6.1.5

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  WB analysis using 86327-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86327-1-RR (CD39 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 86327-1-RR

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 86327-1-RR (CD39 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, Mauslungengewebe, Mausmilzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86327-1-RR bindet in WB, ELISA CD39/ENTPD1 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD39/ENTPD1 fusion protein Eg3483
• Vollständiger Name: ectonucleoside triphosphate diphosphohydrolase 1
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 75 kDa
• GenBank-Zugangsnummer: NM_009848.4
• Gene symbol: Entpd1
• Gene ID (NCBI): 12495
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ENTPD1 (Ectonucleoside triphosphate diphosphohydrolase 1) is also named CD39, NTPDase 1, and Ecto-ATPDase 1 and belongs to the GDA1/CD39 NTPase family. In the nervous system, it can hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. It is a noncovalent tetrameric protein and detergent inhibition of enzymatic activity is caused by dissociation of ectoapyrase tetramers into monomers(PMID: 9733785 ). And it is a 70- to 100-kDa protein that is heavily N-glycosylated (PMID:7930580). This protein has 6 glycosylation sites and 3 isoforms (58 kDa, 59 kDa, and 34 kDa) produced by alternative splicing.