CD39/ENTPD1 Rekombinanter Antikörper

CD39/ENTPD1 Rekombinant Antikörper für FC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

241292G7

Kat-Nr. : 98102-1-PBS

Synonyme

ENTPD1, 241292G7, ATP diphosphohydrolase, ATPDase, ATP-DPH

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  Sandwich ELISA standard curve of MP01143-1

  Sandwich ELISA standard curve of MP01143-1, Human CD39/ENTPD1 Recombinant Matched Antibody Pair - PBS only. 98102-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP02313. 84235-2-PBS was HRP conjugated as the detection antibody. Range: 19.5-1250 pg/mL.

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  Sample test of MP01143-1

  Plasma of six individual healthy human donors was measured. The CD39/ENTPD1 concentration of detected samples was determined to be 179.9 pg/mL with a range of 134.2-243.6 ng/mL.

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  Sample test of MP01143-1

  Serum of six individual healthy human donors was measured. The CD39/ENTPD1 concentration of detected samples was determined to be 373.5 pg/mL with a range of 283.0-450.4 pg/mL.

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  FC experiment of human PBMCs using 98102-1-RR (same clone as 98102-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD39/ENTPD1 Rabbit Recombinant Antibody (98102-1-RR, Clone: 241292G7) or Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were co-stained with CoraLite® Plus 647 Anti-Human CD4 (OKT4). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98102-1-PBS in a different storage buffer formulation.

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  FC experiment of human PBMCs using 98102-1-RR (same clone as 98102-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD39/ENTPD1 Rabbit Recombinant Antibody (98102-1-RR, Clone: 241292G7) or Isotype Control, and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to staining. Cells were not fixed. This data was developed using the same antibody clone with 98102-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01143-1

  Cytometric bead array standard curve of MP01143-1, ENTPD1/CD39 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 98102-1-PBS. Detection antibody: 84235-2-PBS. Standard: RP02313. Range: 0.156-20 ng/mL.

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Geprüfte Anwendungen

Produktinformation

98102-1-PBS bindet in FC, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test CD39/ENTPD1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: ectonucleoside triphosphate diphosphohydrolase 1
• Berechnetes Molekulargewicht: 58 kDa
• GenBank-Zugangsnummer: BC047664
• Gene symbol: CD39
• Gene ID (NCBI): 953
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

ENTPD1 (Ectonucleoside triphosphate diphosphohydrolase 1) is also named CD39, NTPDase 1, and Ecto-ATPDase 1 and belongs to the GDA1/CD39 NTPase family. In the nervous system, it can hydrolyze ATP and other nucleotides to regulate purinergic neurotransmission. It is a noncovalent tetrameric protein and detergent inhibition of enzymatic activity is caused by dissociation of ectoapyrase tetramers into monomers (PMID: 9733785 ).