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CD3 Delta Monoklonaler Antikörper

CD3 Delta Monoklonal Antikörper für WB,ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus

Anwendung

WB,ELISA

Konjugation

Unkonjugiert

CloneNo.

3A12H2

Kat-Nr. : 60194-1-Ig

Synonyme

CD3 delta, CD3D, CD3-delta, T cell receptor T3 delta chain, T3D

Galerie der Validierungsdaten

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  • Western Blot (WB) analysis of jurkat cells using CD3 Delta Monoclonal antibody (60194-1-Ig)

    WB analysis of jurkat using 60194-1-Ig

    jurkat cells were subjected to SDS PAGE followed by western blot with 60194-1-Ig (CD3 delta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Geprüfte Anwendungen

Erfolgreiche Detektion in WBJurkat-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

60194-1-Ig bindet in WB,ELISA CD3 Delta und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Maus / IgG1
Klonalität Monoklonal
Typ Antikörper
Immunogen CD3 Delta fusion protein Ag10207
Vollständiger Name CD3d molecule, delta (CD3-TCR complex)
Berechnetes Molekulargewicht 171 aa, 19 kDa
Beobachtetes Molekulargewicht 25 kDa
GenBank-ZugangsnummerBC070321
Gene symbol CD3D
Gene ID (NCBI) 915
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-G-Reinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells.

Protokolle

Produktspezifische Protokolle
WB protocol for CD3 Delta antibody 60194-1-IgProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen