CD3 Delta Rekombinanter Antikörper

CD3 Delta Rekombinant Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241523H2

Kat-Nr. : 84214-4-PBS

Synonyme

CD3D, 241523H2, CD3-delta, T cell receptor T3 delta chain, T3D

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  WB analysis using 84214-4-RR (same clone as 84214-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84214-4-RR (CD3 Delta antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84214-4-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 84214-4-RR (same clone as 84214-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84214-4-RR (CD3 Delta antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84214-4-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 84214-4-RR (same clone as 84214-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84214-4-RR (CD3 Delta antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84214-4-PBS in a different storage buffer formulation.

• 

  IF Staining of human tonsillitis using 84214-4-RR (same clone as 84214-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using CD3 Delta antibody (84214-4-RR, Clone: 241523H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84214-4-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

84214-4-PBS bindet in WB, IHC, IF-P, Indirect ELISA CD3 Delta und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD3 Delta fusion protein Eg2288
• Vollständiger Name: CD3d molecule, delta (CD3-TCR complex)
• Berechnetes Molekulargewicht: 171 aa, 19 kDa
• Beobachtetes Molekulargewicht: 20-25 kDa
• GenBank-Zugangsnummer: BC070321
• Gene symbol: CD3 Delta
• Gene ID (NCBI): 915
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. CD3 delta/CD3D is a single-pass type I membrane protein which consists of an extracellular domain of 84 amino acids, a transmembrane region, and a cytoplasmic domain of 45 amino acids. Defects in CD3 delta are a cause of severe combined immunodeficiency.