CD3 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

CD3 Polyklonal Antikörper für FC, IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, FC, Cell treatment, ELISA

Konjugation

Unkonjugiert

Publikationen

91

Rezensionen (3)

Kat-Nr. : 17617-1-AP

Synonyme

CD3, CD3 epsilon, CD3E, CD3-epsilon, T3E, TCRE

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Galerie der Validierungsdaten

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  WB analysis using 17617-1-AP

  Jurkat cells (CD3+), MOLT-4 cells (CD3+), U-937 cells (CD3-), Raji cells (CD3-), mouse thymus tissue, and rat thymus tissue were subjected to SDS PAGE followed by western blot with 17617-1-AP (CD3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 17617-1-AP

  WB result of CD3 antibody (17617-1-AP; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CD3 transfected Jurkat cells.

• 

  WB analysis of pig spleen using 17617-1-AP

  pig spleen tissue were subjected to SDS PAGE followed by western blot with 17617-1-AP (CD3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 17617-1-AP

  IP Result of anti-CD3 (IP:17617-1-AP, 4ug; Detection:17617-1-AP 1:1000) with Jurkat cells lysate 3600ug.

• 

  IHC staining of human tonsillitis using 17617-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 17617-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 17617-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 17617-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 17617-1-AP

  Immunohistochemical analysis of paraffin-embedded human angioimmunoblastic T-cell lymphoma using 17617-1-AP (CD3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lymphoma using 17617-1-AP

  Immunohistochemical analysis of paraffin-embedded human angioimmunoblastic T-cell lymphoma using 17617-1-AP (CD3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human breast cancer using 17617-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 17617-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 17617-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 17617-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 17617-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:5000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat thymus using 17617-1-AP

  Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat thymus using 17617-1-AP

  Immunohistochemical analysis of paraffin-embedded rat thymus tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 17617-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 17617-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 17617-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 17617-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 17617-1-AP (CD3 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 17617-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD3 antibody (17617-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CD20 antibody (60271-1-Ig, Clone: 4A7G3, red).

• 

  IF Staining of human tonsillitis using 17617-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD3 antibody (17617-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CD20 antibody (60271-1-Ig, Clone: 4A7G3, red).

• 

  IF Staining of mouse spleen using 17617-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CD3 antibody (17617-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse spleen using 17617-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CD3 antibody (17617-1-AP) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human tonsillitis using 17617-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CD3 antibody (17617-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CD20 antibody (60271-1-Ig, Clone: 4A7G3, red).

• 

  FC experiment of Jurkat using 17617-1-AP

  1X10^6 Jurkat cells were stained with 0.5ug CD3 antibody (17617-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Jurkat-Zellen, Hausschwein-Milzgewebe, Maus-Thymusgewebe, MOLT-4-Zellen, Ratten-Thymusgewebe
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Lungenkarzinomgewebe, humanes Lymphomgewebe, humanes Mammakarzinomgewebe, humanes Pankreaskarzinomgewebe, Maus-Kolongewebe, Mausmilzgewebe, Ratten-Thymusgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Tonsillitisgewebe, Mausmilzgewebe
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 5 publications below
• IHC: See 53 publications below
• IF: See 28 publications below
• FC: See 4 publications below

Produktinformation

17617-1-AP bindet in WB, IP, IHC, IF, FC, Cell treatment, ELISA CD3 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Hausschwein, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD3 fusion protein Ag11797
• Vollständiger Name: CD3e molecule, epsilon (CD3-TCR complex)
• Berechnetes Molekulargewicht: 207 aa, 23 kDa
• Beobachtetes Molekulargewicht: 23-25 kDa
• GenBank-Zugangsnummer: BC049847
• Gene symbol: CD3E
• Gene ID (NCBI): 916
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells. This anti-CD3 is a polyclonal antibody directed against the epsilon chain of human CD3 molecule.

Publikationen

Species	Application	Title
human	IHC	Blood; C1Q labels a highly aggressive macrophage-like leukemia population indicating extramedullary infiltration and relapse; Authors - Li-Xue Yang
human	IHC	Nat Commun; Engineered triple inhibitory receptor resistance improves anti-tumor CAR-T cell performance via CD56.; Authors - Fan Zou
rat	IHC	J Adv Res; Zearalenone-14-glucoside specifically promotes dysplasia of Gut-Associated Lymphoid Tissue: A natural product for constructing intestinal nodular lymphatic hyperplasia model; Authors - Haonan Ruan
human	IHC	Cancer Res; M6A Demethylase ALKBH5 Regulates PD-L1 Expression and Tumor Immunoenvironment in Intrahepatic Cholangiocarcinoma.; Authors - Xinyao Qiu
mouse	IHC	Br J Pharmacol; Allicin ameliorates imiquimod-induced psoriasis-like skin inflammation via disturbing the interaction of keratinocytes with IL-17A; Authors - Lu Zhang
mouse	FC	Bioact Mater; Photosynthetic microorganisms coupled photodynamic therapy for enhanced antitumor immune effect.; Authors - Haoran Wang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Federica (Verified Customer) (12-08-2023)

Leica Bond RXm Red Chromogenic Kit Antigen Retrival ER1 (ph6) for 30 min

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:400
• Cell Tissue Type: Tonsil

CD3 Antibody Immunohistochemistry validation (1:400 dilution) in Tonsil (Cat no:17617-1-AP)

FH

Niklas (Verified Customer) (07-31-2023)

works well

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: human heart

FH

Emma (Verified Customer) (11-29-2021)

This works really well by IF on FFPE tissue. We used a 1:50 dilution and Tris-EDTA antigen retrieval.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Prostate

CD3 Antibody Immunofluorescence validation (1:50 dilution) in Prostate (Cat no:17617-1-AP)

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