• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

CD3EAP Polyklonaler Antikörper

CD3EAP Polyklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31162-1-PBS

Synonyme

Filter:
  • Western Blot (WB) analysis of various lysates using CD3EAP Polyclonal antibody (31162-1-AP)

    WB analysis using 31162-1-AP (same clone as 31162-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31162-1-AP (CD3EAP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31162-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using CD3EAP Polyclonal antibody (31162-1-AP)

    IF Staining of A431 using 31162-1-AP (same clone as 31162-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using CD3EAP antibody (31162-1-AP) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 31162-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

31162-1-PBS bindet in WB, IF/ICC, Indirect ELISA CD3EAP und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen CD3EAP fusion protein Ag35074
Vollständiger Name CD3e molecule, epsilon associated protein
Berechnetes Molekulargewicht 510 aa, 55 kDa
Beobachtetes Molekulargewicht80-90 kDa
GenBank-ZugangsnummerBC038992
Gene symbol CD3EAP
Gene ID (NCBI) 10849
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human RNA polymerase I (Pol I)-specific subunit, previously identified as ASE-1 and as CD3ɛ-associated signal transducer (CAST), CD3EAP is a 55 kDa nucleolar autoantigen that has an apparent molecular mass of 90 kDa (PMID: 11199923). CD3EAP/hPAF49 contains a single tyrosine residue at position 82 (Tyr82), which is phosphorylated upon stimulation of the T-cell receptor. Western blot analysis detected CD3EAP at an apparent molecular mass of ~80-90 kDa.