CD3EAP Rekombinanter Antikörper

CD3EAP Rekombinant Antikörper für WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241379H5

Kat-Nr. : 84139-4-PBS

Synonyme

241379H5, A34.5, Antisense to ERCC-1 protein, ASE1, ASE-1

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  WB analysis using 84139-4-RR (same clone as 84139-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84139-4-RR (CD3EAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84139-4-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 84139-4-RR (same clone as 84139-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using CD3EAP antibody (84139-4-RR, Clone: 241379H5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84139-4-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 84139-4-RR (same clone as 84139-4-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CD3EAP antibody (84139-4-RR, Clone: 241379H5 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84139-4-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 84139-4-RR (same clone as 84139-4-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug CD3EAP Recombinant antibody (84139-4-RR, Clone:241379H5) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84139-4-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01056-3

  Cytometric bead array standard curve of MP01056-3, CD3EAP Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84139-4-PBS. Detection antibody: 84139-2-PBS. Standard: Ag35074. Range: 0.625-80 ng/mL.

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  Affinity and Kinetic Characterization of 84139-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84139-4-RR against Human CD3EAP were performed. The affinity constant is 0.339 nM.

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Geprüfte Anwendungen

Produktinformation

84139-4-PBS bindet in WB, IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA CD3EAP und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD3EAP fusion protein Ag35074
• Vollständiger Name: CD3e molecule, epsilon associated protein
• Berechnetes Molekulargewicht: 510 aa, 55 kDa
• Beobachtetes Molekulargewicht: 80-90 kDa
• GenBank-Zugangsnummer: BC038992
• Gene symbol: CD3EAP
• Gene ID (NCBI): 10849
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Human RNA polymerase I (Pol I)-specific subunit, previously identified as ASE-1 and as CD3ɛ-associated signal transducer (CAST), CD3EAP is a 55 kDa nucleolar autoantigen that has an apparent molecular mass of 90 kDa (PMID: 11199923). CD3EAP/hPAF49 contains a single tyrosine residue at position 82 (Tyr82), which is phosphorylated upon stimulation of the T-cell receptor. Western blot analysis detected CD3EAP at an apparent molecular mass of ~80-90 kDa.