CD3 Gamma Polyklonaler Antikörper

CD3 Gamma Polyklonal Antikörper für FC, IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, FC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 21120-1-AP

Synonyme

CD3 gamma, CD3G, CD3-gamma, T cell receptor T3 gamma chain, T3G

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Galerie der Validierungsdaten

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  WB analysis of mouse thymus using 21120-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 21120-1-AP (CD3 gamma antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 21120-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21120-1-AP (CD3 gamma antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse thymus using 21120-1-AP

  IP Result of anti-CD3 gamma (IP:21120-1-AP, 4ug; Detection:21120-1-AP 1:600) with mouse thymus tissue lysate 2800ug.

• 

  IHC staining of human tonsillitis using 21120-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21120-1-AP (CD3 gamma antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 21120-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 21120-1-AP (CD3 gamma antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 21120-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 21120-1-AP (CD3 gamma antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lymphoma using 21120-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 21120-1-AP (CD3 gamma antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of Jurkat using 21120-1-AP

  1X10^6 Jurkat cells were stained with 0.5ug CD3 gamma antibody (21120-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Thymusgewebe, Jurkat-Zellen
• Erfolgreiche IP: Maus-Thymusgewebe
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in FC: Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

21120-1-AP bindet in WB, IP, IHC, FC, ELISA CD3 Gamma und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD3 Gamma fusion protein Ag15475
• Vollständiger Name: CD3g molecule, gamma (CD3-TCR complex)
• Berechnetes Molekulargewicht: 182 aa, 20 kDa
• Beobachtetes Molekulargewicht: 20-25 kDa
• GenBank-Zugangsnummer: BC113830
• Gene symbol: CD3G
• Gene ID (NCBI): 917
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells.

Publikationen

Species	Application	Title
human	IHC	Cell Mol Gastroenterol Hepatol; Dipeptidyl Peptidase 4 Inhibitors Reduce Hepatocellular Carcinoma by Activating Lymphocyte Chemotaxis in Mice.; Authors - Sohji Nishina
		Life Sci Alliance; Sleep-wake patterns are altered with age, Prdm13 signaling in the DMH, and diet restriction in mice; Authors - Shogo Tsuji

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