CD3 Gamma Monoklonaler Antikörper

CD3 Gamma Monoklonal Antikörper für WB, ELISA

Wirt / Isotyp

Maus / IgG2b

Getestete Reaktivität

human

Anwendung

WB, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

CloneNo.

2E9G7

Kat-Nr. : 60347-1-Ig

Synonyme

CD3G, 2E9G7, CD3-gamma, T cell receptor T3 gamma chain, T3G

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  WB analysis using 60347-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60347-1-Ig (CD3 Gamma antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MOLT-4-Zellen, Jurkat-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 2 publications below
• IF: See 1 publications below

Produktinformation

60347-1-Ig bindet in WB, IHC, IF, ELISA CD3 Gamma und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD3 Gamma fusion protein Ag15502
• Vollständiger Name: CD3g molecule, gamma (CD3-TCR complex)
• Berechnetes Molekulargewicht: 182 aa, 20 kDa
• Beobachtetes Molekulargewicht: 20-25 kDa
• GenBank-Zugangsnummer: BC113830
• Gene symbol: CD3 Gamma
• Gene ID (NCBI): 917
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD3 is a complex of proteins that directly associates with the T cell receptor (TCR). The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. The TCR recognizes antigens bound to major histocompatibility complex (MHC) molecules. TCR-mediated peptide-MHC recognition is transmitted to the CD3 complex, leading to the intracellular signal transduction. CD3 is considered to be a pan-T cell marker for detection of normal and neoplastic T cells.

Publikationen

Species	Application	Title
human	IHC	Cancer Sci; Metformin changes the immune microenvironment of colorectal cancer in patients with type 2 diabetes mellitus.; Authors - Akira Saito
human	IF	J Breast Cancer; Metformin Alters Tumor Immune Microenvironment, Improving the Outcomes of Breast Cancer Patients With Type 2 Diabetes Mellitus; Authors - Satomi Shiba
human	IHC	J Anus Rectum Colon; Reduced Abundance of Phocaeicola in Mucosa-associated Microbiota Is Associated with Distal Colorectal Cancer Metastases Possibly through an Altered Local Immune Environment; Authors - Gaku Ota