CD4 Polyklonaler Antikörper

CD4 Polyklonal Antikörper für IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen

23

Rezensionen (1)

Kat-Nr. : 19068-1-AP

Synonyme

CD4, CD4 molecule, CD4mut

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Galerie der Validierungsdaten

• 

  WB analysis using 19068-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 19068-1-AP (CD4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 19068-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 19068-1-AP (CD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig thymus using 19068-1-AP

  pig thymus tissue were subjected to SDS PAGE followed by western blot with 19068-1-AP (CD4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse thymus using 19068-1-AP

  IP Result of anti-CD4 (IP:19068-1-AP, 4ug; Detection:19068-1-AP 1:500) with mouse thymus tissue lysate 2800ug.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: U-937-Zellen, Hausschwein-Thymusgewebe, Maus-Thymusgewebe
• Erfolgreiche IP: Maus-Thymusgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 7 publications below

Produktinformation

19068-1-AP bindet in WB, IP, ELISA CD4 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD4 fusion protein Ag13616
• Vollständiger Name: CD4 molecule
• Berechnetes Molekulargewicht: 55 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC025782
• Gene symbol: CD4
• Gene ID (NCBI): 920
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells. CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation. CD4 serves as a receptor for the human immunodeficiency virus (HIV).

Publikationen

Species	Application	Title
mouse		ACS Appl Mater Interfaces; Fe(III)-Chelated Polydopamine Nanoparticles for Synergistic Tumor Therapies of Enhanced Photothermal Ablation and Antitumor Immune Activation.; Authors - Na Xu
mouse	WB	J Control Release; Cancer-cell-biomimetic Upconversion nanoparticles combining chemo-photodynamic therapy and CD73 blockade for metastatic triple-negative breast cancer.; Authors - Feiyang Jin
human		J Hematol Oncol; PD-1 axis expression in musculoskeletal tumors and antitumor effect of nivolumab in osteosarcoma model of humanized mouse.; Authors - Bingxin Zheng
rat		Int J Mol Sci; Human Milk Oligosaccharide 2'-Fucosyllactose Induces Neuroprotection from Intracerebral Hemorrhage Stroke.; Authors - Tsai-Wei Hung
rat		Exp Neurol; Calpain mediated expansion of CD4+ cytotoxic T cells in rodent models of Parkinson's disease.; Authors - Azizul Haque
mouse		Food Funct; Genistein suppresses allergic contact dermatitis through regulating the MAP2K2/ERK pathway.; Authors - Jinhong Xu

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Nicole (Verified Customer) (04-28-2022)

no signal expression is controlled by other antibodies

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Hela, Plasmid driven expression of various CD4 fusion constructs

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