CD4 Monoklonaler Antikörper

CD4 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

IF, FC

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

RPA-T4

Rezensionen (3)

Kat-Nr. : 65143-1-Ig

Synonyme

CD4 molecule, CD4mut, RPA-T4, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

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• 

  FC experiment of human PBMCs using 65143-1-Ig

  1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD4 (65143-1-Ig, Clone:RPA-T4) or Mouse IgG1 Isotype Control and Multi-rAb CoraLite ® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM005) at dilution 1:1000. Cells were then stained with CL488-conjugated Anti-Human CD3 (CL488-65151). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 65143-1-Ig

  1x10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD4 (65143-1-Ig, Clone:RPA-T4) or Mouse IgG1 Isotype Control and Multi-rAb CoraLite ® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM005) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

65143-1-Ig bindet in IF, FC CD4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: CD4 molecule
• Berechnetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC025782
• Gene symbol: CD4
• Gene ID (NCBI): 920
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).

Publikationen

Species	Application	Title
human	IF	Allergy Asthma Immunol Res; TET2 Regulates 5-Hydroxymethylcytosine Signature and CD4+ T-Cell Balance in Allergic Rhinitis.; Authors - Lu Tan

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sunil (Verified Customer) (08-03-2023)

worked as expected for conjugating our custom dyes to

• Applications: Flow Cytometry

FH

Sareena (Verified Customer) (06-02-2023)

Worked well with compensation beads

• Applications: Immunofluorescence, Flow Cytometry

FH

Sareena (Verified Customer) (04-20-2023)

Worked well with conjugating fluorophores and tested with compensation beads for flow cytometry analysis.

• Applications: Flow Cytometry
• Primary Antibody Dilution: 100 ug
• Cell Tissue Type: CD4 RPA-T4 antibody