CD4 Monoklonaler Antikörper

CD4 Monoklonal Antikörper für FC, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

FC, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

SK3

Kat-Nr. : 65147-1-PBS

Synonyme

CD4 molecule, CD4mut, SK3, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

---

• 

  Sandwich ELISA standard curve of MP50077-1

  Sandwich ELISA standard curve of MP50077-1, CD4 Monoclonal Matched Antibody Pair - PBS only. 65147-1-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard  Eg0333. 68688-1-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

• 

  Sample test of MP50077-1

  The mean CD4 concentration was determined to be 5.16 ng/mL in HL-60 cell extract based on a 4.3 mg/mL extract load.

• 

  Sample test of MP50077-1

  Serum of sixteen individual healthy human donors was measured. The human CD4 concentration of detected samples was determined to be 101.4 pg/mL with a range of 12.7 - 255.0 pg/mL.

• 

  FC experiment of human PBMCs using 65147-1-Ig (same clone as 65147-1-PBS)

  1X10^6 human PBMCs were surface stained with CoraLite® Plus 488 Anti-Human CD3 and 0.2 ug Anti-Human CD4 (65147-1-Ig, Clone:SK3) labeled with FlexAble CoraLite® Plus 750 Antibody Labeling Kit for Mouse IgG1 (KFA024) (right), or Mouse IgG1 Isotype Control (left). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65147-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65147-1-Ig (same clone as 65147-1-PBS)

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD4 (65147-1-Ig, Clone:SK3) labeled with FlexAble CoraLite® Plus 750 Antibody Labeling Kit for Mouse IgG1 (KFA024) (red), or Mouse IgG1 Isotype Control (blue). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65147-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

65147-1-PBS bindet in FC, Sandwich ELISA, Indirect ELISA, Sample test CD4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: CD4 molecule
• Berechnetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC025782
• Gene symbol: CD4
• Gene ID (NCBI): 920
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).