CD4 Rekombinanter Antikörper

CD4 Rekombinant Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

250263G12

Kat-Nr. : 86300-3-RR

Synonyme

CD4 antigen, L3T4, Ly 4, T-cell differentiation antigen L3T4, T-cell surface antigen T4/Leu-3

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  WB analysis using 86300-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86300-3-RR (CD4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 86300-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 86300-3-RR (CD4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 86300-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 86300-3-RR (CD4 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse thymus using 86300-3-RR

  Immunohistochemical analysis of paraffin-embedded mouse thymus tissue slide using 86300-3-RR (CD4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse spleen using 86300-3-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse spleen tissue using CD4 antibody (86300-3-RR, Clone: 250263G12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse thymus using 86300-3-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse thymus tissue using CD4 antibody (86300-3-RR, Clone: 250263G12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse thymus using 86300-3-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse thymus tissue using CD4 antibody (86300-3-RR, Clone: 250263G12 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Thymusgewebe, Mausmilzgewebe
• Erfolgreiche Detektion in IHC: Mausmilzgewebe, Maus-Thymusgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: Maus-Thymusgewebe, Mausmilzgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)-P: IF-P : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86300-3-RR bindet in WB, IHC, IF-P, ELISA CD4 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD4 fusion protein Eg3847
• Vollständiger Name: CD4 antigen
• Berechnetes Molekulargewicht: 51 kDa
• Beobachtetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: NM_013488
• Gene symbol: Cd4
• Gene ID (NCBI): 12504
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, the majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).