CD4 Rekombinanter Antikörper

CD4 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human, nicht-humane Primaten

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

240427E12

Kat-Nr. : 98042-1-RR

Synonyme

240427E12, CD4 molecule, CD4mut, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

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  FC experiment of human PBMCs using 98042-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD4 Rabbit RecAb (98042-1-RR, Clone: 240427E12) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were not fixed. Lymphocytes were gated.

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  FC experiment of Cnyo PBMCs using 98042-1-RR

  1x10^6 cynomolgus PBMCs were surface stained with 0.25 ug Anti-Human CD4 Rabbit RecAb (98042-1-RR, Clone:240427E12) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98042-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD4 Rabbit RecAb (98042-1-RR, Clone: 240427E12) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of Cnyo PBMCs using 98042-1-RR

  1x10^6 cynomolgus PBMCs were surface stained with 0.25 ug Anti-Human CD4 Rabbit RecAb (98042-1-RR, Clone:240427E12) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-conjugated Goat Anti-Rabbit IgG. Cells were not fixed.

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  Affinity and Kinetic Characterization of 98042-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98042-1-RR against Human CD4 were performed. The affinity constant is 3.88 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs, cynomolgus PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98042-1-RR bindet in FC CD4 und zeigt Reaktivität mit Affe, human, nicht-humane Primaten

• Getestete Reaktivität: Affe, human, nicht-humane Primaten
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD4 fusion protein Eg0333
• Vollständiger Name: CD4 molecule
• Berechnetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC025782
• Gene symbol: CD4
• Gene ID (NCBI): 920
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, the majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).