CD4 Rekombinanter Antikörper

CD4 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Affe, human, nicht-humane Primaten

Anwendung

FC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240427E12

Kat-Nr. : CL488-98042

Synonyme

240427E12, CD4 molecule, CD4mut, T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

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  FC experiment of human PBMCs using CL488-98042

  1x10^6 human PBMCs were surface stained with CoraLite® Plus 647 Anti-Human CD3 and 5 ul CoraLite® Plus 488 Anti-Human CD4 Rab bit Recombinant Antibody (CL488-98042, Clone:240427E12)(right), or Isotype Control(left). Cells were not fixed. Lymphocytes were gated.

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  FC experiment of human PBMCs using CL488-98042

  1x10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD4 Rabbit Recombinant Antibody (CL488-98042, Clone:240427E12)(red), or Isotype Control(blue). Cells were not fixed. Lymphocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-98042 bindet in FC CD4 und zeigt Reaktivität mit Affe, human, nicht-humane Primaten

• Getestete Reaktivität: Affe, human, nicht-humane Primaten
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD4 fusion protein Eg0333
• Vollständiger Name: CD4 molecule
• Berechnetes Molekulargewicht: 55 kDa
• GenBank-Zugangsnummer: BC025782
• Gene symbol: CD4
• Gene ID (NCBI): 920
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD4 is a 55-kDa transmembrane glycoprotein expressed on T helper cells, the majority of thymocytes, monocytes, macrophages, and dendritic cells (PMID: 9304802; 12213222). CD4 is an accessory protein for MHC class-II antigen/T-cell receptor interaction. It plays an important role in T helper cell development and activation (PMID: 9539765; 3112582). CD4 serves as a receptor for the human immunodeficiency virus (HIV) (PMID: 9304802).