CD40 Monoklonaler Antikörper

CD40 Monoklonal Antikörper für FC, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

FC, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

G28.5

Kat-Nr. : 65103-1-PBS

Synonyme

B cell surface antigen CD40, B-cell surface antigen CD40, Bp50, CD40L receptor, G28.5

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  Sandwich ELISA standard curve of MP50064-1

  Sandwich ELISA standard curve of MP50064-1, CD40 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66965-2-PBS. Detection antibody: 65103-1-PBS. Standard: Ag28040. Range: 78.1-5000 pg/mL.

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  Sample test of MP50064-1

  Urine of eight individual healthy human donors was measured. The CD40 concentration of detected samples was determined to be 1,053.4 pg/mL with a range of 79.9-2,169.7 pg/mL.

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  Sample test of MP50064-1

  Saliva of six individual healthy human donors was measured. The CD40 concentration of detected samples was determined to be 684.6 pg/mL with a range of 349.9-1,498.5 pg/mL.

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  Sample test of MP50064-1

  Human milk of six individual healthy human donors was measured. The CD40 concentration of detected samples was determined to be 53.4 pg/mL with a range of 29.8-104.3 pg/mL.

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  Sample test of MP50064-1

  Plasma of fifteen individual healthy human donors was measured. The CD40 concentration of detected samples was determined to be 89.6 pg/mL with a range of 39.1-155.7 pg/mL.

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  FC experiment of human PBMCs using 65103-1-Ig (same clone as 65103-1-PBS)

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD40 (65103-1-Ig, Clone:G28.5) or Mouse IgG1 Isotype Control and CoraLite®647-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were then stained with CoraLite®488 Anti-Human CD19. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65103-1-PBS in a different storage buffer formulation.

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  FC experiment of human PBMCs using 65103-1-Ig (same clone as 65103-1-PBS)

  1X10^6 human PBMCs were surface stained with 0.2 ug Anti-Human CD40 (65103-1-Ig, Clone:G28.5) or Mouse IgG1 Isotype Control and CoraLite®647-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65103-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

65103-1-PBS bindet in FC, Sandwich ELISA, Indirect ELISA, Sample test CD40 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: CD40 molecule, TNF receptor superfamily member 5
• Berechnetes Molekulargewicht: 277 aa, 31 kDa
• GenBank-Zugangsnummer: BC012419
• Gene symbol: CD40
• Gene ID (NCBI): 958
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

CD40, also named as TNFRSF5 and Bp50, is a type I transmembrane protein that belongs to the tumor necrosis factor-R (TNF-R) family (PMID: 10647992). CD40 is expressed on B cells, dendritic cells (DCs), monocytes, platelets, and macrophages as well as by non-hematopoietic cells such as myofibroblasts, fibroblasts, epithelial, and endothelial cells (PMID: 19426221). It is a receptor for TNFSF5/CD40LG. CD40L/CD40 interactions are essential for immunoglobulin (Ig) isotype switching, germinal center formation and the development of B cell memory (PMID: 7516405; 11675497).