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CD40 Rekombinanter Antikörper

CD40 Rekombinant Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

240874B2

Kat-Nr. : 83972-5-PBS

Synonyme

Tnfrsf5, CD40 antigen, Bp50, 240874B2

Filter:
  • Western Blot (WB) analysis of various lysates using CD40 Recombinant antibody (83972-5-RR)

    WB analysis using 83972-5-RR (same clone as 83972-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83972-5-RR (CD40 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83972-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83972-5-RR

    Affinity and Kinetic Characterization of 83972-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83972-5-RR against Mouse CD40 were performed. The affinity constant is 4.50 nM.

Geprüfte Anwendungen

Produktinformation

83972-5-PBS bindet in WB, Indirect ELISA CD40 und zeigt Reaktivität mit Maus

Getestete Reaktivität Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen Rekombinantes Protein
Vollständiger Name CD40 antigen
Berechnetes Molekulargewicht32 kDa
Beobachtetes Molekulargewicht40-45 kDa
GenBank-ZugangsnummerNM_011611.2
Gene symbol Cd40
Gene ID (NCBI) 21939
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cluster of differentiation 40 (CD40) is a costimulatory protein located on antigen presenting cells and is required for their activation. CD40 is a member of the tumor necrosis factor (TNF) receptor (TNFR) family.

What is the molecular weight of CD40?

The molecular weight of CD40 is 43 kDa.

What is the cellular localization of CD40?

CD40 can be secreted by cells or found in the cell membrane.

What is the tissue specificity of CD40?

CD40 is expressed in B cells and primary carcinoma cells but is also found in dendritic cells and macrophages (PMID: 10209159).

What is the function of CD40?

CD40 acts as a receptor for TNFSF5/CD40LG, which is expressed on activated T cells. This interaction is essential for B cell proliferation, expression of activation markers, immunoglobulin production, and isotype switching (PMID: 8809473). This interaction is also crucial for the formation of memory B cells and germinal centers, and signaling through CD40 prevents apoptosis of germinal center B cells.

What is the role of CD40 in disease?

Defects in CD40 lead to hyper-IgM immunodeficiency syndrome type 3 (HIGM3) (PMID: 11675497). This is an autosomal recessive disorder that includes the inability of B cells to undergo isotype switching, a key step in the final differentiation of the humoral immune response, and an inability to mount an antibody-specific immune response.