CD40 Rekombinanter Antikörper

CD40 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

240874G8

Kat-Nr. : 98122-1-RR

Synonyme

Tnfrsf5, CD40 antigen, Bp50, 240874G8

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  FC experiment of mouse splenocytes using 98122-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD40 Rabbit Recombinant Antibody (98122-1-RR, Clone: 240874G8) or 0.25 ug Isotype Control, and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were co-stained with APC Anti-Mouse CD19 (1D3) (APC-65290, Clone: 1D3). Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 98122-1-RR

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD40 Rabbit Recombinant Antibody (98122-1-RR, Clone: 240874G8) (red) or 0.25 ug Isotype Control (blue) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  Affinity and Kinetic Characterization of 98122-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98122-1-RR against Mouse CD40 were performed. The affinity constant is 0.997 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98122-1-RR bindet in FC CD40 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: CD40 antigen
• Berechnetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: NM_011611.2
• Gene symbol: Cd40
• Gene ID (NCBI): 21939
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

CD40, also named as TNFRSF5 and Bp50, is a type I transmembrane protein that belongs to the tumor necrosis factor-R (TNF-R) family (PMID: 10647992). CD40 is expressed on B cells, dendritic cells (DCs), monocytes, platelets, and macrophages as well as by non-hematopoietic cells such as myofibroblasts, fibroblasts, epithelial, and endothelial cells (PMID: 19426221). It is a receptor for TNFSF5/CD40LG. CD40L/CD40 interactions are essential for immunoglobulin (Ig) isotype switching, germinal center formation and the development of B cell memory (PMID: 7516405; 11675497).