CD40 Rekombinanter Antikörper

CD40 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

APC Fluorescent Dye

CloneNo.

241760A9

Kat-Nr. : APC-98191

Synonyme

241760A9, B cell surface antigen CD40, B-cell surface antigen CD40, Bp50, CD40L receptor

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  FC experiment of human PBMCs using APC-98191

  1x10^6 human PBMCs were surface stained with CoraLite® Plus 488 Anti-Human CD19, and 5 ul APC Anti-Human CD40 Rabbit RecAb (APC-98191, Clone: 241760A9) or APC Rabbit IgG Isotype Control RecAb (APC-98136, Clone: 240953C9). Cells were not fixed. Lymphocytes were gated.

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  FC experiment of human PBMCs using APC-98191

  1x10^6 human PBMCs were surface stained with 5 ul APC Anti-Human CD40 Rabbit RecAb (APC-98191, Clone: 241760A9) (red) or APC Rabbit IgG Isotype Control RecAb (APC-98136, Clone: 240953C9) (blue). Cells were not fixed. Lymphocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

APC-98191 bindet in FC CD40 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD40 fusion protein Eg1797
• Vollständiger Name: CD40 molecule, TNF receptor superfamily member 5
• Berechnetes Molekulargewicht: 31kDa
• GenBank-Zugangsnummer: NM_001250.6
• Gene symbol: CD40
• Gene ID (NCBI): 958
• Konjugation: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Cluster of differentiation 40 (CD40) is a costimulatory protein located on antigen presenting cells and is required for their activation. CD40 is a member of the tumor necrosis factor (TNF) receptor (TNFR) family.

What is the molecular weight of CD40?

The molecular weight of CD40 is 43 kDa.

What is the cellular localization of CD40?

CD40 can be secreted by cells or found in the cell membrane.

What is the tissue specificity of CD40?

CD40 is expressed in B cells and primary carcinoma cells but is also found in dendritic cells and macrophages (PMID: 10209159).

What is the function of CD40?

CD40 acts as a receptor for TNFSF5/CD40LG, which is expressed on activated T cells. This interaction is essential for B cell proliferation, expression of activation markers, immunoglobulin production, and isotype switching (PMID: 8809473). This interaction is also crucial for the formation of memory B cells and germinal centers, and signaling through CD40 prevents apoptosis of germinal center B cells.

What is the role of CD40 in disease?

Defects in CD40 lead to hyper-IgM immunodeficiency syndrome type 3 (HIGM3) (PMID: 11675497). This is an autosomal recessive disorder that includes the inability of B cells to undergo isotype switching, a key step in the final differentiation of the humoral immune response, and an inability to mount an antibody-specific immune response.