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CD40L/CD154 Rekombinanter Antikörper

CD40L/CD154 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240743F5

Kat-Nr. : 83884-3-PBS

Synonyme

CD154, Cd40l, 240743F5, CD40 ligand, Cd40lg

Filter:
  • Western Blot (WB) analysis of various lysates using CD40L/CD154 Recombinant monoclonal antibody (83884-3-RR)

    WB analysis using 83884-3-RR (same clone as 83884-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83884-3-RR (CD40L/CD154 antibody) at dilution of 1:9000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83884-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83884-3-RR

    Affinity and Kinetic Characterization of 83884-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83884-3-RR against Mouse CD40L/CD154 were performed. The affinity constant is 0.406 nM.

Geprüfte Anwendungen

Produktinformation

83884-3-PBS bindet in WB, ELISA CD40L/CD154 und zeigt Reaktivität mit Maus

Getestete Reaktivität Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen CD40L/CD154 fusion protein Eg0990
Vollständiger Name CD40 ligand
Berechnetes Molekulargewicht29 kDa
Beobachtetes Molekulargewicht32 kDa
GenBank-ZugangsnummerNM_011616.2
Gene symbol CD154
Gene ID (NCBI) 21947
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33 kDa transmembrane homologue or an 18 kDa soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. Recent studies have suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis.