CD40L/CD154 Rekombinanter Antikörper

CD40L/CD154 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

240743F5

Kat-Nr. : 83884-3-RR

Synonyme

CD154, Cd40l, 240743F5, CD40 ligand, Cd40lg

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  WB analysis using 83884-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83884-3-RR (CD40L/CD154 antibody) at dilution of 1:9000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83884-3-RR

  Biolayer interferometry (BLl) kinetic assays of 83884-3-RR against Mouse CD40L/CD154 were performed. The affinity constant is 0.406 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: J774A.1 cells, CTLL-2 cells

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:18000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83884-3-RR bindet in WB, ELISA CD40L/CD154 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD40L/CD154 fusion protein Eg0990
• Vollständiger Name: CD40 ligand
• Berechnetes Molekulargewicht: 29 kDa
• Beobachtetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: NM_011616.2
• Gene symbol: CD154
• Gene ID (NCBI): 21947
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33 kDa transmembrane homologue or an 18 kDa soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. Recent studies have suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis.