CD40/TNFRSF5 Rekombinanter Antikörper

CD40/TNFRSF5 Rekombinant Antikörper für Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240874A9

Kat-Nr. : 83972-1-PBS

Synonyme

Cd40, Bp50, CD40 antigen, Tnfrsf5

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  Sandwich ELISA standard curve of MP00896-1

  Sandwich ELISA standard curve of MP00896-1, Mouse CD40 Recombinant Matched Antibody Pair - PBS only. 83972-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg1118. 83972-1-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL

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  Sample test of MP00896-1

  Serum of sixteen mice was measured. The mouse CD40 concentration of detected samples was determined to be 122.5 pg/mL with a range of 10.4 - 236.8 pg/mL.

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  Sample test of MP00896-1

  The mean CD40 concentration was determined to be 23,443.1 pg/mL in mouse spleen tissue extract based on a 3.0 mg/mL extract load, 390.2 pg/mL in mouse heart tissue extract based on a 11.2 mg/mL extract load, 216.2 pg/mL in mouse kidney tissue extract based on a 1.5 mg/mL extract load, 3,196.8 pg/mL in mouse liver tissue extract based on a 3.0 mg/mL extract load and 9,508.1 pg/mL in mouse lung tissue extract based on a 3.0 mg/mL extract load.

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  Sample test of MP00896-1

  Mouse spleen tissue, mouse kidney tissue and mouse lung tissue was cultured for 3 days in RPMI supplemented with 10% fetal bovine serum, 5 μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin, 100 μg/mL streptomycin sulfate, and stimulated with 1 μg/mL LPS. The mean CD40 concentration was determined to be 5.7 pg/mL, 37.2 pg/mL and 8.3 pg/mL in unstimulated mouse spleen tissue, mouse kidney tissue and mouse lung tissue supernatant, 6.2 pg/mL, 26.2 pg/mL and 4.5 pg/mL in LPS stimulated mouse spleen tissue, mouse kidney tissue and mouse lung tissue supernatant.

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  Cytometric bead array standard curve of MP00896-1

  Cytometric bead array standard curve of MP00896-1, MOUSE CD40 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83972-2-PBS. Detection antibody: 83972-1-PBS. Standard: Eg1118. Range: 0.313- 40 ng/mL.

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Geprüfte Anwendungen

Produktinformation

83972-1-PBS bindet in Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test CD40/TNFRSF5 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD40/TNFRSF5 fusion protein Eg1118
• Vollständiger Name: CD40 antigen
• Berechnetes Molekulargewicht: 32 kDa
• GenBank-Zugangsnummer: NM_011611.2
• Gene symbol: Cd40
• Gene ID (NCBI): 21939
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.