CD40 Rekombinanter Antikörper

CD40 Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241760A4

Kat-Nr. : 84406-1-PBS

Synonyme

241760A4, B cell surface antigen CD40, B-cell surface antigen CD40, Bp50, CD40L receptor

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  WB analysis using 84406-1-RR (same clone as 84406-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84406-1-RR (CD40/TNFRSF5 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84406-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 84406-1-RR (same clone as 84406-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84406-1-RR (CD40 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84406-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 84406-1-RR (same clone as 84406-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84406-1-RR (CD40 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84406-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

84406-1-PBS bindet in WB, IHC, Indirect ELISA CD40 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD40 fusion protein Eg1797
• Vollständiger Name: CD40 molecule, TNF receptor superfamily member 5
• Berechnetes Molekulargewicht: 31kDa
• Beobachtetes Molekulargewicht: 40-45 kDa
• GenBank-Zugangsnummer: NM_001250.6
• Gene symbol: CD40
• Gene ID (NCBI): 958
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cluster of differentiation 40 (CD40) is a costimulatory protein located on antigen presenting cells and is required for their activation. CD40 is a member of the tumor necrosis factor (TNF) receptor (TNFR) family.

What is the molecular weight of CD40?

The molecular weight of CD40 is 43 kDa.

What is the cellular localization of CD40?

CD40 can be secreted by cells or found in the cell membrane.

What is the tissue specificity of CD40?

CD40 is expressed in B cells and primary carcinoma cells but is also found in dendritic cells and macrophages (PMID: 10209159).

What is the function of CD40?

CD40 acts as a receptor for TNFSF5/CD40LG, which is expressed on activated T cells. This interaction is essential for B cell proliferation, expression of activation markers, immunoglobulin production, and isotype switching (PMID: 8809473). This interaction is also crucial for the formation of memory B cells and germinal centers, and signaling through CD40 prevents apoptosis of germinal center B cells.

What is the role of CD40 in disease?

Defects in CD40 lead to hyper-IgM immunodeficiency syndrome type 3 (HIGM3) (PMID: 11675497). This is an autosomal recessive disorder that includes the inability of B cells to undergo isotype switching, a key step in the final differentiation of the humoral immune response, and an inability to mount an antibody-specific immune response.