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CD40 Rekombinanter Antikörper

CD40 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241760A11

Kat-Nr. : 84406-2-PBS

Synonyme

241760A11, B cell surface antigen CD40, B-cell surface antigen CD40, Bp50, CD40L receptor

Filter:
  • Western Blot (WB) analysis of various lysates using CD40 Recombinant antibody (84406-2-RR)

    WB analysis using 84406-2-RR (same clone as 84406-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84406-2-RR (CD40/TNFRSF5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84406-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using CD40 Recombinant antibody (84406-2-RR)

    IF Staining of Raji using 84406-2-RR (same clone as 84406-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using CD40/TNFRSF5 antibody (84406-2-RR, Clone: 241760A11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84406-2-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

84406-2-PBS bindet in WB, IF/ICC, Indirect ELISA CD40 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen CD40 fusion protein Eg1797
Vollständiger Name CD40 molecule, TNF receptor superfamily member 5
Berechnetes Molekulargewicht31kDa
Beobachtetes Molekulargewicht45 kDa
GenBank-ZugangsnummerNM_001250.6
Gene symbol CD40
Gene ID (NCBI) 958
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Cluster of differentiation 40 (CD40) is a costimulatory protein located on antigen presenting cells and is required for their activation. CD40 is a member of the tumor necrosis factor (TNF) receptor (TNFR) family.

What is the molecular weight of CD40?

The molecular weight of CD40 is 43 kDa.

What is the cellular localization of CD40?

CD40 can be secreted by cells or found in the cell membrane.

What is the tissue specificity of CD40?

CD40 is expressed in B cells and primary carcinoma cells but is also found in dendritic cells and macrophages (PMID: 10209159).

What is the function of CD40?

CD40 acts as a receptor for TNFSF5/CD40LG, which is expressed on activated T cells. This interaction is essential for B cell proliferation, expression of activation markers, immunoglobulin production, and isotype switching (PMID: 8809473). This interaction is also crucial for the formation of memory B cells and germinal centers, and signaling through CD40 prevents apoptosis of germinal center B cells.

What is the role of CD40 in disease?

Defects in CD40 lead to hyper-IgM immunodeficiency syndrome type 3 (HIGM3) (PMID: 11675497). This is an autosomal recessive disorder that includes the inability of B cells to undergo isotype switching, a key step in the final differentiation of the humoral immune response, and an inability to mount an antibody-specific immune response.