CD40L/CD154 Monoklonaler Antikörper

CD40L/CD154 Monoklonal Antikörper für WB, Indirect ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human, Ratte

Anwendung

WB, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1E6D10

Kat-Nr. : 66502-1-PBS

Synonyme

CD154, CD40 L, CD40 ligand, CD40L, CD40L/CD154, CD40LG, gp39, hCD40L, HIGM1, IGM, IMD3, T BAM, T cell antigen Gp39, TNF related activation protein, TNFSF5, TRAP

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  WB analysis of Jurkat using 66502-1-Ig (same clone as 66502-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66502-1-Ig (CD40L/CD154 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66502-1-PBS in a different storage buffer formulation.

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  WB analysis of human peripheral blood platelets using 66502-1-Ig (same clone as 66502-1-PBS)

  human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 66502-1-Ig (CD40L/CD154 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66502-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66502-1-PBS bindet in WB, Indirect ELISA CD40L/CD154 und zeigt Reaktivität mit human, Ratten

• Getestete Reaktivität: human, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD40L/CD154 fusion protein Ag24941
• Vollständiger Name: CD40 ligand
• Berechnetes Molekulargewicht: 261 aa, 29 kDa
• Beobachtetes Molekulargewicht: 30 kDa
• GenBank-Zugangsnummer: BC071754
• Gene symbol: CD40 Ligand
• Gene ID (NCBI): 959
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33-kd transmembrane homologue or 18-kd soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and on a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. Recent studies have suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis. Its expression is tightly regulated, and abnormal levels of CD40L are associated with the pathogenesis of atheromatous plaque destabilization and thrombotic events. Multiple mutations in CD40LG gene have been identified that are associated with hyper-IgM immunodeficiency syndrome type 1.