CD40L/CD154 Rekombinanter Antikörper

CD40L/CD154 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

242847F7

Kat-Nr. : 98355-2-PBS

Synonyme

CD154, CD40L; CD154, CD40LG, CD 154, CD 40L

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  FC experiment of human PBMCs using 98355-2-RR (same clone as 98355-2-PBS)

  1x10^6 untreated or PMA and ionomycin-treated human PBMCs were surface stained with 0.25 ug Anti-Human CD40L/CD154 Rabbit RecAb (98355-2-RR, Clone: 242847F7) and PE-conjugated Goat Anti-Rabbit IgG. Cells were co-stained with APC Anti-Human CD4. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98355-2-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 98355-2-RR (same clone as 98355-2-PBS)

  1x10^6 PMA and ionomycin treated human PBMCs were surface stained with 0.25 ug Anti-Human CD40L/CD154 Rabbit RecAb (98355-2-RR, Clone:242847F7) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-conjugated Goat Anti-Rabbit IgG. Untreated human PBMCs were surface stained with 0.25 ug Anti-Human CD40L/CD154 Rabbit RecAb (98355-2-RR, Clone: 242847F7) and PE-conjugated Goat Anti-Rabbit IgG (black, dashed). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 98355-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

98355-2-PBS bindet in FC CD40L/CD154 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: CD40 ligand
• Berechnetes Molekulargewicht: 261 aa, 29 kDa
• GenBank-Zugangsnummer: BC071754
• Gene symbol: CD40 Ligand
• Gene ID (NCBI): 959
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33 kDa transmembrane homologue or 18 kDa soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and on a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. Recent studies have suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis.