CD40L/CD154 Rekombinanter Antikörper

CD40L/CD154 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

242847F7

Kat-Nr. : 98355-2-RR

Synonyme

CD154, CD40L; CD154, CD40LG, CD 154, CD 40L

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  FC experiment of human PBMCs using 98355-2-RR

  1x10^6 untreated or PMA and ionomycin-treated human PBMCs were surface stained with 0.25 ug Anti-Human CD40L/CD154 Rabbit RecAb (98355-2-RR, Clone: 242847F7) and PE-conjugated Goat Anti-Rabbit IgG. Cells were co-stained with APC Anti-Human CD4. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using 98355-2-RR

  1x10^6 PMA and ionomycin treated human PBMCs were surface stained with 0.25 ug Anti-Human CD40L/CD154 Rabbit RecAb (98355-2-RR, Clone:242847F7) (red) or Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue) and PE-conjugated Goat Anti-Rabbit IgG. Untreated human PBMCs were surface stained with 0.25 ug Anti-Human CD40L/CD154 Rabbit RecAb (98355-2-RR, Clone: 242847F7) and PE-conjugated Goat Anti-Rabbit IgG (black, dashed). Cells were not fixed. Lymphocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: PMA and ionomycin treated human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98355-2-RR bindet in FC CD40L/CD154 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: CD40 ligand
• Berechnetes Molekulargewicht: 261 aa, 29 kDa
• GenBank-Zugangsnummer: BC071754
• Gene symbol: CD40 Ligand
• Gene ID (NCBI): 959
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33 kDa transmembrane homologue or 18 kDa soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and on a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. Recent studies have suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis.