CD40L/CD154 Rekombinanter Antikörper

CD40L/CD154 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

242847F7

Kat-Nr. : CL750-98355-2

Synonyme

CD154, CD40L; CD154, CD40LG, CD 154, CD 40L

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  FC experiment of human PBMCs using CL750-98355-2

  1x10^6 untreated or PMA and ionomycin treated human PBMCs were surface stained with CoraLite® Plus 488 Anti-Human CD4 and 5 ul CoraLite® Plus 750 Anti-Human CD40L/CD154 Rabbit RecAb (CL750-98355-2, Clone: 242847F7). Cells were not fixed.

• 

  FC experiment of human PBMCs using CL750-98355-2

  1x10^6 PMA and ionomycin treated human PBMCs were surface stained with 5 ul CoraLite® Plus 750 Anti-Human CD40L/CD154 Rabbit RecAb (CL750-98355-2, Clone: 242847F7) (red) or CoraLite® Plus 750 Rabbit IgG Isotype Control RecAb (CL750-98136, Clone: 240953C9) (blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul CoraLite® Plus 750 Anti-Human CD40L/CD154 Rabbit RecAb (CL750-98355-2, Clone: 242847F7) (black, dashed). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: PMA and ionomycin treated human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL750-98355-2 bindet in FC CD40L/CD154 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD40L/CD154 fusion protein Eg3501
• Vollständiger Name: CD40 ligand
• Berechnetes Molekulargewicht: 261 aa, 29 kDa
• GenBank-Zugangsnummer: BC071754
• Gene symbol: CD40 Ligand
• Gene ID (NCBI): 959
• Konjugation: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 755 nm / 780 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33 kDa transmembrane homologue or 18 kDa soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and on a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. Recent studies have suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis.