CD40L/CD154 Rekombinanter Antikörper

CD40L/CD154 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

PE-Cyanine5.5 Fluorescent Dye

CloneNo.

240743E6

Kat-Nr. : PY5P5-FcA98120

Synonyme

CD154, Cd40l, 240743E6, CD40 ligand, Cd40lg

---

• 

  FC experiment of mouse CD3+ T cells using PY5P5-FcA98120

  1x10^6 untreated or PMA and ionomycin treated mouse CD3+ T cells were surface stained with FcZero-rAb™ APC Anti-Mouse CD4 (RM4-4) and 0.25 ug PE-Cyanine5.5 Anti-Mouse CD40L/CD154 Rabbit RecAb (PY5P5-FcA98120, Clone:240743E6). Cells were incubated with MonoZero™ Monocytes blocking prior to staining. Cells were not fixed.

• 

  FC experiment of mouse CD3+ T cells using PY5P5-FcA98120

  1x10^6 PMA and ionomycin treated mouse CD3+ T cells were surface stained with 0.25 ug PE-Cyanine5.5 Anti-Mouse CD40L/CD154 Rabbit RecAb (PY5P5-FcA98120, Clone: 240743E6) (red) or FcZero-rAb™ PE-Cyanine5.5 Rabbit IgG Isotype Control Recombinant Antibody (PY5P5-FcA98136, Clone: 240953C9) (blue). 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug PE-Cyanine5.5 Anti-Mouse CD40L/CD154 Rabbit RecAb (PY5P5-FcA98120, Clone: 240743E6) (black, dashed). Cells were incubated with MonoZero™ Monocytes blocking prior to staining. Cells were not fixed.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: PMA and ionomycin treated mouse CD3+ T cells

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

PY5P5-FcA98120 bindet in FC CD40L/CD154 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD40L/CD154 fusion protein Eg0990
• Vollständiger Name: CD40 ligand
• Berechnetes Molekulargewicht: 29 kDa
• GenBank-Zugangsnummer: NM_011616.2
• Gene symbol: CD154
• Gene ID (NCBI): 21947
• Konjugation: PE-Cyanine5.5 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 488 nm, 561 nm / 692 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33-kd transmembrane homologue or 18-kd soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and on a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. It has been suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis. Its expression is tightly regulated, and abnormal levels of CD40L are associated with the pathogenesis of atheromatous plaque destabilization and thrombotic events. Multiple mutations in CD40LG gene have been identified that are associated with hyper-IgM immunodeficiency syndrome type 1.