CD40L/CD154 Polyklonaler Antikörper

CD40L/CD154 Polyklonal Antikörper für WB, IHC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(14)

Rezensionen (1)

Kat-Nr. : 16668-1-AP

Synonyme

CD154, CD40L; CD154, CD40LG, CD 154, CD 40L

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  WB analysis of mouse small intestine using 16668-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 16668-1-AP (CD40LG antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 16668-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16668-1-AP (CD40LG antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16668-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16668-1-AP (CD40L; CD154 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 16668-1-AP

  IP result of anti-CD40L/CD154 (IP:16668-1-AP, 4ug; Detection:16668-1-AP 1:1000) with Jurkat cells lysate 3200ug.

• 

  IHC staining of human tonsillitis using 16668-1-AP

  Immunohistochemical analysis of paraffin-embedded tonsillitis slide using 16668-1-AP (CD40L/CD154 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human lung cancer using 16668-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung cancer tissue using CD40L/CD154 antibody (16668-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human lung cancer using 16668-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human lung cancer tissue using CD40L/CD154 antibody (16668-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Dünndarmgewebe, HeLa-Zellen, Jurkat-Zellen
• Erfolgreiche IP: Jurkat-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Lungenkarzinomgewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 9 publications below
• IHC: See 5 publications below

Produktinformation

16668-1-AP bindet in WB, IHC, IF-P, IP, ELISA CD40L/CD154 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: CD40L/CD154 fusion protein Ag10147
• Vollständiger Name: CD40 ligand
• Berechnetes Molekulargewicht: 261 aa, 29 kDa
• Beobachtetes Molekulargewicht: 29-39 kDa
• GenBank-Zugangsnummer: BC071754
• Gene symbol: CD40 Ligand
• Gene ID (NCBI): 959
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

The CD40 ligand (CD40L, TRAP, CD154), a member of the TNF superfamily of ligands, is expressed as either a 33 kDa transmembrane homologue or 18 kDa soluble form (sCD154). CD40L is primarily expressed on activated CD4+ T cells and on a small proportion of CD8+ T cells and platelets. It binds to CD40 on antigen-presenting cells (APC), which leads to many effects depending on the target cell type. Recent studies have suggested that CD40/CD40L interactions regulate oxidative stress and affect various signaling pathways in both the immunological and the cardiovascular systems. The CD40/CD40L system is also involved in tumorigenesis.

Publikationen

Species	Application	Title
mouse	WB	Sci Adv; Promoting the activation of T cells with glycopolymer-modified dendritic cells by enhancing cell interactions.; Authors - Liyin Yu
mouse	IHC	J Mol Cell Cardiol; TNF-α promotes early atherosclerosis by increasing transcytosis of LDL across endothelial cells: crosstalk between NF-κB and PPAR-γ; Authors - Youzhi Zhang
mouse	IHC	Br J Pharmacol; CRP promotes atherosclerosis by increasing LDL transcytosis across endothelial cells.; Authors - Fang Bian
human	WB	Pharmacol Res; PKCβII-mediated cross-talk of TRPV1/CB2 modulates the glucocorticoid-induced osteoclast overactivity.; Authors - Giulia Bellini
rat	IHC	Sci China Life Sci; Compression loading of osteoclasts attenuated microRNA-146a-5p expression, which promotes angiogenesis by targeting adiponectin.; Authors - Yue Wang
mouse	WB	Bone; YAP1 is essential for osteoclastogenesis through a TEADs-dependent mechanism.; Authors - Liming Zhao

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sara (Verified Customer) (10-10-2024)

It worked well in immunofluorence, the staining colocalised with the CD3+ cells (T cells) but not with B cells.

• Applications: Immunofluorescence

CD40L/CD154 Antibody Immunofluorescence validation ( dilution) in (Cat no:16668-1-AP)