CD41 Monoklonaler Antikörper

CD41 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human, nicht-humane Primaten

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

HIP8

Kat-Nr. : 65173-1-Ig

Synonyme

CD41a, CD41B, GP2B, GPalpha IIb, GPIIb

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  FC experiment of human peripheral blood using 65173-1-Ig

  100 ul human platelet rich plasma (diluted 1:4 with PBS) was surface stained with 0.2 ug Anti-Human CD41a (65173-1-Ig, Clone:HIP8) or Mouse IgG1 Isotype Control (65124-1-Ig, Clone: MOPC-21) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM005). Cells were not fixed.

• 

  FC experiment of human peripheral blood using 65173-1-Ig

  100 ul human platelet rich plasma (diluted 1:4 with PBS) was surface stained with 0.2 ug Anti-Human CD41a (65173-1-Ig, Clone:HIP8) or Mouse IgG1 Isotype Control (65124-1-Ig, Clone: MOPC-21) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM005). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: human peripheral blood

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

65173-1-Ig bindet in FC CD41 und zeigt Reaktivität mit human, nicht-humane Primaten

• Getestete Reaktivität: human, nicht-humane Primaten
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41)
• GenBank-Zugangsnummer: BC117443
• Gene symbol: CD41
• Gene ID (NCBI): 3674
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID: 19118207). Integrin alpha-IIb (ITGA2B, CD41) is expressed on platelets, megakaryocytes and some hematopoietic progenitor cells (PMID: 11934866). This protein undergoes post-translational cleavage to yield disulfide linked light beta (25 kDa) and heavy alpha (125 kDa) chains, which join with integrin beta3 (CD61) to form a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. CD41/CD61 is crucial for platelet aggregation through binding of soluble fibrinogen. The HIP8 clone antibody specifically binds to the alpha chain of CD41.