CD41 Monoklonaler Antikörper

CD41 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human, nicht-humane Primaten

Anwendung

FC

Konjugation

Atlantic Blue™ Fluorescent Dye

CloneNo.

HIP8

Kat-Nr. : AB-65173

Synonyme

CD41a, CD41B, GP2B, GPalpha IIb, GPIIb

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  FC experiment of human peripheral blood platelets using AB-65173

  100 ul human platelet-rich plasma (diluted 1:2 with PBS) was surface stained with 5 ul Atlantic Blue™ Anti-Human CD41a (AB-65173, Clone: HIP8) or Atlantic Blue™ Mouse IgG1 Isotype Control (AB-65124). Cells were not fixed.

• 

  FC experiment of human peripheral blood platelets using AB-65173

  100 ul human platelet-rich plasma (diluted 1:2 with PBS) was surface stained with 5 ul Atlantic Blue™ Anti-Human CD41a (AB-65173, Clone:HIP8) or Atlantic Blue™ Mouse IgG1 Isotype Control (AB-65124). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: human peripheral blood platelets

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

AB-65173 bindet in FC CD41 und zeigt Reaktivität mit human, nicht-humane Primaten

• Getestete Reaktivität: human, nicht-humane Primaten
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: integrin, alpha 2b (platelet glycoprotein IIb of IIb/IIIa complex, antigen CD41)
• GenBank-Zugangsnummer: BC117443
• Gene symbol: CD41
• Gene ID (NCBI): 3674
• Konjugation: Atlantic Blue™ Fluorescent Dye
• Excitation/Emission maxima wavelengths: 404 nm / 458 nm
• Form: Liquid
• Reinigungsmethode: Unknow
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

The integrins are a superfamily of cell adhesion receptors that bind to extracellular matrix ligands, cell-surface ligands, and soluble ligands (PMID: 17543136). They are transmembrane αβ heterodimers and at least 24 distinct integrin heterodimers are formed by the combination of 18 α and eight β known subunits (PMID: 17543136; 20029421). In addition to mediating cell adhesion, integrins also play important roles in modulating signal transduction pathways that control cellular responses including migration, proliferation, differentiation, and apoptosis (PMID: 19118207). Integrin alpha-IIb (ITGA2B, CD41) is expressed on platelets, megakaryocytes and some hematopoietic progenitor cells (PMID: 11934866). This protein undergoes post-translational cleavage to yield disulfide linked light beta (25 kDa) and heavy alpha (125 kDa) chains, which join with integrin beta3 (CD61) to form a receptor for fibronectin, fibrinogen, plasminogen, prothrombin, thrombospondin and vitronectin. CD41/CD61 is crucial for platelet aggregation through binding of soluble fibrinogen. The HIP8 clone antibody specifically binds to the alpha chain of CD41.