CD44 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

CD44 Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, IP, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human und mehr (2)

Anwendung

WB, IHC, IF/ICC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(78)

CloneNo.

6F4H2

Rezensionen (1)

Kat-Nr. : 60224-1-Ig

Synonyme

6F4H2, CD44 antigen, CDW44, CSPG8, ECMR III

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  WB analysis using 60224-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60224-1-Ig (CD44 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

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  WB analysis using 60224-1-Ig

  HeLa (CD44+), A549 (CD44+), MDA-MB-231 (CD44+), MCF-7, Jurkat cell lysates were subjected to SDS PAGE followed by western blot with 60224-1-Ig (CD44 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa cells using 60224-1-Ig

  WB result of CD44 antibody (60224-1-Ig, 1:4000) with si-Control and si-CD44 transfected HeLa cells.

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  WB analysis of human spleen using 60224-1-Ig

  human spleen tissue were subjected to SDS PAGE followed by western blot with 60224-1-Ig (CD44 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 60224-1-Ig

  IP result of anti-CD44 (IP:60224-1-Ig, 4ug; Detection:60224-1-Ig 1:3000) with HeLa cells lysate 1280 ug.

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  IHC staining of human lung cancer using 60224-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 60224-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 60224-1-Ig

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60224-1-Ig (CD44 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human oesophagus cancer using 60224-1-Ig

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60224-1-Ig (CD44 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 60224-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60224-1-Ig (CD44 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60224-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 60224-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60224-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 60224-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human colon cancer using 60224-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using CD44 antibody (60224-1-Ig, Clone: 6F4H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human colon cancer using 60224-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using CD44 antibody (60224-1-Ig, Clone: 6F4H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 60224-1-Ig

  Immunofluorescent analysis of un-fixed A431 cells using CD44 antibody (60224-1-Ig, Clone: 6F4H2 ) at dilution of 1:1800 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ).

• 

  IF Staining of A431 using 60224-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CD44 antibody (60224-1-Ig, Clone: 6F4H2 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MDA-MB-231-Zellen, A549-Zellen, HeLa-Zellen, humanes Milzgewebe, HUVEC-Zellen, Hausschwein-Milzgewebe, U-251-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Mammakarzinomgewebe, humanes Ösophaguskarzinomgewebe, humanes Tonsillitisgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Kolonkarzinomgewebe
• Erfolgreiche Detektion in IF/ICC: A431-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:200-1:1000
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 40 publications below
• IHC: See 10 publications below
• IF: See 38 publications below
• ELISA: See 1 publications below
• FC: See 2 publications below

Produktinformation

60224-1-Ig bindet in WB, IHC, IF/ICC, IF-P, IP, ELISA CD44 und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• In Publikationen genannte Reaktivität: human, Hausschwein, Kaninchen, Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD44 fusion protein Ag7633
• Vollständiger Name: CD44 molecule (Indian blood group)
• Berechnetes Molekulargewicht: 742 aa, 82 kDa
• Beobachtetes Molekulargewicht: 80-95 kDa
• GenBank-Zugangsnummer: BC004372
• Gene symbol: CD44
• Gene ID (NCBI): 960
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands. Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. This protein exists in multiple forms (with molecular weight ranging from 16 kDa to over 200 kDa) generated by alternative RNA splicing and extensive post-translational modifications.

Publikationen

Species	Application	Title
	IF	Nature; Cell transcriptomic atlas of the non-human primate Macaca fascicularis.; Authors - Lei Han
human	IF	Nat Methods; Visualizing the native cellular organization by coupling cryofixation with expansion microscopy (Cryo-ExM).; Authors - Marine H Laporte
		Clin Transl Med; PADI4 facilitates stem-like properties and cisplatin resistance through upregulating PRMT2/IDs family in oesophageal squamous cell carcinoma; Authors - Zeyu Wang
pig	IF	Int J Biol Macromol; A light-cured injectable composite hydrogel based on chitosan and decellularized matrix modulates stem cell aggregation behavior for accelerating cartilage defect repair; Authors - Peipei Feng
human	IF	Biomater Sci; Magnetothermal heating facilitates the cryogenic recovery of stem cell-laden alginate-Fe(3)O(4) nanocomposite hydrogels; Authors - Xiaozhang Zhang
human	IF	Biomater Sci; Vitrification of stem cell-laden core-shell microfibers with unusually low concentrations of cryoprotective agents; Authors - Conghui Tian

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Udesh (Verified Customer) (08-16-2023)

Worked well for WB at 1:1000

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Glioblastoma cells