CD44 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

CD44 Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, IP, ELISA

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Hausschwein, human

Anwendung

WB, IHC, IF/ICC, IF-P, IP, ELISA

Konjugation

Unkonjugiert

CloneNo.

6F4H2

Kat-Nr. : 60224-1-PBS

Synonyme

6F4H2, CD44 antigen, CDW44, CSPG8, ECMR III

---

• 

  WB analysis using 60224-1-Ig (same clone as 60224-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60224-1-Ig (CD44 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60224-1-Ig (same clone as 60224-1-PBS)

  HeLa (CD44+), A549 (CD44+), MDA-MB-231 (CD44+), MCF-7, Jurkat cell lysates were subjected to SDS PAGE followed by western blot with 60224-1-Ig (CD44 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa cells using 60224-1-Ig (same clone as 60224-1-PBS)

  WB result of CD44 antibody (60224-1-Ig, 1:4000) with si-Control and si-CD44 transfected HeLa cells. This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human spleen using 60224-1-Ig (same clone as 60224-1-PBS)

  human spleen tissue were subjected to SDS PAGE followed by western blot with 60224-1-Ig (CD44 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 60224-1-Ig (same clone as 60224-1-PBS)

  IP result of anti-CD44 (IP:60224-1-Ig, 4ug; Detection:60224-1-Ig 1:3000) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 60224-1-Ig (same clone as 60224-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 60224-1-Ig (same clone as 60224-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human oesophagus cancer using 60224-1-Ig (same clone as 60224-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60224-1-Ig (CD44 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human oesophagus cancer using 60224-1-Ig (same clone as 60224-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 60224-1-Ig (CD44 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 60224-1-Ig (same clone as 60224-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 60224-1-Ig (CD44 antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60224-1-Ig (same clone as 60224-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 60224-1-Ig (same clone as 60224-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60224-1-Ig (same clone as 60224-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human breast cancer using 60224-1-Ig (same clone as 60224-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 60224-1-Ig (CD44 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human colon cancer using 60224-1-Ig (same clone as 60224-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using CD44 antibody (60224-1-Ig, Clone: 6F4H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human colon cancer using 60224-1-Ig (same clone as 60224-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon cancer tissue using CD44 antibody (60224-1-Ig, Clone: 6F4H2 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 60224-1-Ig (same clone as 60224-1-PBS)

  Immunofluorescent analysis of un-fixed A431 cells using CD44 antibody (60224-1-Ig, Clone: 6F4H2 ) at dilution of 1:1800 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 60224-1-Ig (same clone as 60224-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CD44 antibody (60224-1-Ig, Clone: 6F4H2 ) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002). This data was developed using the same antibody clone with 60224-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

60224-1-PBS bindet in WB, IHC, IF/ICC, IF-P, IP, ELISA CD44 und zeigt Reaktivität mit Hausschwein, human

• Getestete Reaktivität: Hausschwein, human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: CD44 fusion protein Ag7633
• Vollständiger Name: CD44 molecule (Indian blood group)
• Berechnetes Molekulargewicht: 742 aa, 82 kDa
• Beobachtetes Molekulargewicht: 80-95 kDa
• GenBank-Zugangsnummer: BC004372
• Gene symbol: CD44
• Gene ID (NCBI): 960
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD44 is a type I transmembrane glycoprotein that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands. Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis. This protein exists in multiple forms (with molecular weight ranging from 16 kDa to over 200 kDa) generated by alternative RNA splicing and extensive post-translational modifications.