CD44 Monoklonaler Antikörper

CD44 Monoklonal Antikörper für IF/ICC, FC

Wirt / Isotyp

Ratte / IgG2b, kappa

Getestete Reaktivität

human, Maus

Anwendung

IHC, IF/ICC, FC

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

IM7

Kat-Nr. : 65117-1-Ig

Synonyme

CD44, CD44 antigen, CDW44, CSPG8, ECMR III, Epican, HCELL, Heparan sulfate proteoglycan, Hermes antigen, HUTCH I, Hyaluronate receptor, IN, LHR, MC56, MDU2, MDU3, MIC4, MUTCH I, PGP 1, PGP I, Pgp1, Phagocytic glycoprotein 1, Phagocytic glycoprotein I

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  IF Staining of HeLa using 65117-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CD44 antibody (65117-1-Ig, Clone: IM7 ) at dilution of 1:400 and Rhodamine (TRITC)-conjugated Goat Anti-Rat IgG(H+L).

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  FC experiment of C57BL/c mouse splenocytes using 65117-1-Ig

  1X10^6 C57BL/c mouse splenocytes were surface co-stained with APC Anti-Mouse CD3 (17A2), and 0.5 ug Anti-Mouse CD44 (65117-1-Ig, Clone: IM7) or 0.5 ug isotype control, and FITC anti-rat IgG2b Antibody at dilution 1:100. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen
• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:400

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

65117-1-Ig bindet in IHC, IF/ICC, FC CD44 und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Ratte / IgG2b, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Dexamethason-induzierte myeloische Leukämie-M1-Zellen
• Vollständiger Name: CD44 antigen
• GenBank-Zugangsnummer: BC051388
• Gene symbol: Cd44
• Gene ID (NCBI): 12505
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.

Publikationen

Species	Application	Title
	IHC,IF	Adv Sci (Weinh); NETs-CD44-IL-17A Feedback Loop Drives Th17-Mediated Inflammation in Behçet's Uveitis; Authors - Yi Wu