CD44 Rekombinanter Antikörper

CD44 Rekombinant Antikörper für FC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

F10-44-2

Kat-Nr. : 65608-1-PBS

Synonyme

Epican, ECMR-III, ECMR III, CSPG8, CDW44

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  FC experiment of human PBMCs using 65608-1-MR (same clone as 65608-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD44 Mouse IgG2a Recombinant Antibody (65608-1-MR, Clone: F10-44-2) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65608-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65608-1-MR (same clone as 65608-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD44 Mouse IgG2a Recombinant Antibody (65608-1-MR, Clone: F10-44-2) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65608-1-PBS in a different storage buffer formulation.

• 

  FC experiment of human PBMCs using 65608-1-MR (same clone as 65608-1-PBS)

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human CD44 Mouse IgG2a Recombinant Antibody (65608-1-MR, Clone:F10-44-2) (left) or 0.25 ug Anti-Human CD44 (65063-1-Ig, Clone: F10-44-2) (right) and CoraLite®488-Conjugated AffiniPure Donkey Anti-Mouse IgG(H+L). Cells were then stained with CoraLite® Plus 647 Anti-Human CD3. Cells were not fixed. Lymphocytes were gated. This data was developed using the same antibody clone with 65608-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

65608-1-PBS bindet in FC CD44 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: CD44 molecule (Indian blood group)
• Berechnetes Molekulargewicht: 742 aa, 82 kDa
• GenBank-Zugangsnummer: BC004372
• Gene symbol: CD44
• Gene ID (NCBI): 960
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.