CD44 Rekombinanter Antikörper

CD44 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

241648D1

Kat-Nr. : 84369-5-PBS

Synonyme

Hermes antigen, GP90 lymphocyte homing/adhesion receptor, Extracellular matrix receptor III, ECMR-III, CD44 antigen

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  WB analysis of rat spleen using 84369-5-RR (same clone as 84369-5-PBS)

  rat spleen tissue was subjected to SDS PAGE followed by western blot with 84369-5-RR (CD44 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84369-5-PBS in a different storage buffer formulation.

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  IHC staining of rat spleen using 84369-5-RR (same clone as 84369-5-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84369-5-RR (CD44 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84369-5-PBS in a different storage buffer formulation.

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  IHC staining of rat spleen using 84369-5-RR (same clone as 84369-5-PBS)

  Immunohistochemical analysis of paraffin-embedded rat spleen tissue slide using 84369-5-RR (CD44 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84369-5-PBS in a different storage buffer formulation.

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  IF Staining of NR8383 using 84369-5-RR (same clone as 84369-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed NR8383 cells using CD44 antibody (84369-5-RR, Clone: 241648D1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84369-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 84369-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84369-5-RR against Rat CD44 were performed. The affinity constant is 0.332 nM.

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Geprüfte Anwendungen

Produktinformation

84369-5-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CD44 und zeigt Reaktivität mit Ratten

• Getestete Reaktivität: Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: Cd44 molecule
• Berechnetes Molekulargewicht: 40 kDa
• Beobachtetes Molekulargewicht: 80 kDa
• GenBank-Zugangsnummer: XM_006234631.3
• Gene symbol: Cd44
• Gene ID (NCBI): 25406
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.