CD44 Rekombinanter Antikörper

CD44 Rekombinant Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242345G11

Kat-Nr. : 84854-5-PBS

Synonyme

242345G11, CD44 antigen, CDW44, CSPG8, ECMR III

---

• 

  WB analysis using 84854-5-RR (same clone as 84854-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84854-5-RR (CD44 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84854-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 84854-5-RR (same clone as 84854-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84854-5-RR (CD44 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84854-5-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 84854-5-RR (same clone as 84854-5-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 84854-5-RR (CD44 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84854-5-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 84854-5-RR (same clone as 84854-5-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CD44 antibody (84854-5-RR, Clone: 242345G11 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 84854-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 84854-5-PBS

  Biolayer interferometry (BLl) kinetic assay of 84854-5-PBS against Human CD44 was performed. The affinity constant is 2.04 nM.

• 

  Affinity and Kinetic Characterization of 84854-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84854-5-RR against Human CD44 were performed. The affinity constant is 1.01 nM.

---

Geprüfte Anwendungen

Produktinformation

84854-5-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA CD44 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD44 fusion protein Eg1057
• Vollständiger Name: CD44 molecule (Indian blood group)
• Berechnetes Molekulargewicht: 82 kDa
• Beobachtetes Molekulargewicht: 80-95 kDa
• GenBank-Zugangsnummer: NM_000610.3
• Gene symbol: CD44
• Gene ID (NCBI): 960
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.