CD44 Monoklonaler Antikörper

CD44 Monoklonal Antikörper für FC, IF

Wirt / Isotyp

Ratte / IgG2b, kappa

Getestete Reaktivität

human, Maus

Anwendung

IF, FC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

IM7

Kat-Nr. : CL488-65117

Synonyme

AU023126, AW121933, AW146109, Cd44, CD44 antigen, HERMES, Ly 24, Pgp 1



Geprüfte Anwendungen

Erfolgreiche Detektion in IFMaus-Splenozyten
Erfolgreiche Detektion in FCMaus-Splenozyten

Empfohlene Verdünnung

AnwendungVerdünnung
Immunfluoreszenz (IF)IF : 1:50-1:500
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL488-65117 bindet in IF, FC CD44 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Ratte / IgG2b, kappa
Klonalität Monoklonal
Typ Antikörper
Immunogen Dexamethason-induzierte myeloische Leukämie-M1-Zellen
Vollständiger Name CD44 antigen
GenBank-ZugangsnummerBC051388
Gene symbol Cd44
Gene ID (NCBI) 12505
Konjugation CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Reinigungsmethode Affinitätsreinigung
Lagerungspuffer PBS mit 0,1% Natriumazid und 0,5% BSA, pH 7,3.
LagerungsbedingungenStore at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.

Protokolle

Produktspezifische Protokolle
IF protocol for CL Plus 488 CD44 antibody CL488-65117Protokoll herunterladen
FC protocol for CL Plus 488 CD44 antibody CL488-65117Protokoll herunterladen
Standard-Protokolle
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