CD44 Monoklonaler Antikörper

CD44 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG2a, kappa

Getestete Reaktivität

human

Anwendung

IF, FC

Konjugation

FITC Plus Fluorescent Dye

Publikationen(5)

CloneNo.

F10-44-2

Kat-Nr. : FITC-65063

Synonyme

Epican, ECMR-III, ECMR III, CSPG8, CDW44

---

• 

  FC experiment of human PBMCs using FITC-65063

  1x10^6 human PBMCs were surface stained with APC Anti-Human CD3, and 5 ul FITC Plus Anti-Human CD44 (F10-44-2) (FITC-65063, Clone: F10-44-2) or FITC Plus Mouse IgG2a Isotype Control (C1.18.4) (FITC-65208, Clone: C1.18.4). Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using FITC-65063

  1x10^6 human PBMCs were surface stained with 5 ul FITC Plus Anti-Human CD44 (F10-44-2) (FITC-65063, Clone: F10-44-2) (red) or FITC Plus Mouse IgG2a Isotype Control (C1.18.4) (FITC-65208, Clone: C1.18.4) (blue). Cells were not fixed. Lymphocytes were gated.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below
• FC: See 4 publications below

Produktinformation

FITC-65063 bindet in IF, FC CD44 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Gereinigte T-Zellen aus humanen Lymphknoten
• Vollständiger Name: CD44 molecule (Indian blood group)
• Berechnetes Molekulargewicht: 742 aa, 82 kDa
• GenBank-Zugangsnummer: BC004372
• Gene symbol: CD44
• Gene ID (NCBI): 960
• Konjugation: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD44 is a type I transmembrane glycoprotein expressed on embryonic stem cells and in various levels on other cell types including connective tissues and bone marrow. CD44 expression is also upregulated in subpopulations of cancer cells and is recognized as a molecular marker for cancer stem cells (PMID: 29747682). It is a cell-surface receptor that mediates cell-cell and cell-matrix interactions through its affinity for hyaluronic acid (HA) and possibly also through its affinity for other ligands (PMID: 10694938). Adhesion with HA plays an important role in cell migration, tumor growth and progression. CD44 is also involved in lymphocyte activation, recirculation and homing, and in hematopoiesis.

Publikationen

Species	Application	Title
human	FC	ACS Nano; Inhibition Effect of Ti3C2Tx MXene on Ice Crystals Combined with Laser-Mediated Heating Facilitates High-Performance Cryopreservation.; Authors - Yuan Cao
human	IF	Free Radic Biol Med; Retinal cell-targeted liposomal ginsenoside Rg3 attenuates retinal ischemia-reperfusion injury via alleviating oxidative stress and promoting microglia/macrophage M2 polarization; Authors - Yanmei Huang
human	FC	Hum Cell; Replication factor C4, which is regulated by insulin-like growth factor 2 mRNA binding protein 2, enhances the radioresistance of breast cancer by promoting the stemness of tumor cells; Authors - Xiao-Yu Zhu
human	FC	J Ethnopharmacol; Jinwu Jiangu Capsule Alleviates Rheumatoid Arthritis Symptoms by Regulating the ADCY10 and cAMP/RANKL Pathways; Authors - Qiuyi Wang
human	FC	Open Life Sci; Experimental study on the optimization of ANM33 release in foam cells; Authors - Chen Yuan