CD45 Monoklonaler Antikörper

CD45 Monoklonal Antikörper für FC

Wirt / Isotyp

Ratte / IgG2b, kappa

Getestete Reaktivität

Maus

Anwendung

IHC, FC

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

30-F11

Kat-Nr. : 65087-1-Ig

Synonyme

Ptprc, 30-F11, EC:3.1.3.48, LCA, L-CA

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  FC experiment of mouse splenocytes using 65087-1-Ig

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD45 (30-F11) (65087-1-Ig, Clone: 30-F11) or 0.25 ug Rat IgG2b Isotype Control (LTF-2) (65211-1-Ig, Clone: LTF-2), and FITC anti-rat IgG2b Antibody. Cells were not fixed.

• 

  FC experiment of mouse splenocytes using 65087-1-Ig

  1x10^6 mouse splenocytes were surface stained with 0.25 ug Anti-Mouse CD45 (30-F11) (65087-1-Ig, Clone:30-F11) or 0.25 ug Rat IgG2b Isotype Control (LTF-2) (65211-1-Ig, Clone: LTF-2), and FITC anti-rat IgG2b Antibody. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IHC: See 1 publications below

Produktinformation

65087-1-Ig bindet in IHC, FC CD45 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2b, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Maus-Thymus oder -milz
• Vollständiger Name: protein tyrosine phosphatase, receptor type, C
• GenBank-Zugangsnummer: BC028512
• Gene symbol: CD45
• Gene ID (NCBI): 19264
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

Publikationen

Species	Application	Title
mouse	IHC	JCI Insight; NAD+ prevents chronic kidney disease by activating renal tubular metabolism; Authors - Bryce A Jones