CD45 Monoklonaler Antikörper

CD45 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG2a, kappa

Getestete Reaktivität

human

Anwendung

FC

Konjugation

APC Fluorescent Dye

Publikationen

2

CloneNo.

F10-89-4

Kat-Nr. : APC-65064

Synonyme

B220, CD45, CD45R, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200

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Galerie der Validierungsdaten

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  FC experiment of human PBMCs using APC-65064

  1X10^6 human PBMCs were surface stained with 10 ul APC Anti-Human CD45 (APC-65064, Clone: F10-89-4) or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

• 

  FC experiment of human PBMCs using APC-65064

  1X10^6 human PBMCs were surface stained with 10 ul APC Anti-Human CD45 (APC-65064, Clone: F10-89-4) or Mouse IgG2a Isotype Control. Cells were not fixed. Lymphocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 10 µl per 10^6 cells in a 100 µl suspension or 10 µl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• FC: See 2 publications below

Produktinformation

APC-65064 bindet in FC CD45 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Gereinigte T-Zellen aus humanen Lymphknoten
• Vollständiger Name: protein tyrosine phosphatase, receptor type, C
• GenBank-Zugangsnummer: BC014239
• Gene symbol: PTPRC
• Gene ID (NCBI): 5788
• Konjugation: APC Fluorescent Dye
• Excitation/Emission maxima wavelengths: 650 nm / 660 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS mit 0,1% Natriumazid und einem Stabilisator.
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

Publikationen

Species	Application	Title
	FC	Asian J Pharm Sci; Bone mesenchymal stem cell-derived exosomes involved co-delivery and synergism effect with icariin via mussel-inspired multifunctional hydrogel for cartilage protection; Authors - Jia Zeng
human	FC	Microsyst Nanoeng; From the teapot effect to tap-triggered self-wetting: a 3D self-driving sieve for whole blood filtration; Authors - Yuang Li