CD45 Monoklonaler Antikörper

CD45 Monoklonal Antikörper für IF/ICC, FC

Wirt / Isotyp

Maus / IgG1, kappa

Getestete Reaktivität

human

Anwendung

IF/ICC, FC

Konjugation

CoraLite® Plus 488 Fluorescent Dye

Publikationen(1)

CloneNo.

2D1

Kat-Nr. : CL488-65082

Synonyme

2D1, EC:3.1.3.48, GP180, L CA, LCA

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  IF Staining of human peripheral blood mononuclear cells using CL488-65082

  Immunofluorescent analysis of human peripheral blood mononuclear cells (PBMCs) using CoraLite® Plus 488-conjugated Anti-Human CD45 (CL488-65082, Clone: 2D1) at dilution of 1:400.

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  FC experiment of human PBMCs using CL488-65082

  1X10^6 human PBMCs were surface stained with 5 ul CoraLite® Plus 488 Anti-Human CD45 (CL488-65082, Clone:2D1) (red), or 5 ul Control Antibody. Cells were not fixed .

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: humane mononukleäre Zellen aus peripherem Blut
• Erfolgreiche Detektion in FC: humane PBMCs

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 µl per 10^6 cells in a 100 µl suspension or 5 µl per 100 µl of whole blood.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

CL488-65082 bindet in IF/ICC, FC CD45 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Maus / IgG1, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Humanes PBMC
• Vollständiger Name: protein tyrosine phosphatase, receptor type, C
• GenBank-Zugangsnummer: BC014239
• Gene symbol: CD45
• Gene ID (NCBI): 5788
• Konjugation: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).

Publikationen

Species	Application	Title
human	IF	Anal Chim Acta; Layer-by-layer fabrication of alginate/polyethyleneimine multilayer on magnetic interface with enhanced efficiency in immuno-capturing circulating tumor cells; Authors - Yang Xiao