CD45 Rekombinanter Antikörper

CD45 Rekombinant Antikörper für FC (Intra), IF

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

IF, FC (Intra)

Konjugation

CoraLite®555 Fluorescent Dye

CloneNo.

6O19

Rezensionen (1)

Kat-Nr. : CL555-80297

Synonyme

B220, CD45, CD45R, GP180, L CA, LCA, Leukocyte common antigen, LY5, PTPRC, RPTPC, T200

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Galerie der Validierungsdaten

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  IF Staining of mouse spleen using CL555-80297

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CoraLite®555 CD45 antibody (CL555-80297, Clone: 6O19 ) at dilution of 1:200.

• 

  IF Staining of mouse spleen using CL555-80297

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CoraLite®555 CD45 antibody (CL555-80297, Clone: 6O19 ) at dilution of 1:200.

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  FC experiment of Raji using CL555-80297

  1X10^6 Raji cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human CD45 (CL555-80297, Clone:6O19) (red), or 0.4 ug x. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF: Mausmilzgewebe
• Erfolgreiche Detektion in FC: Raji-Zellen

Empfohlene Verdünnung

• Immunfluoreszenz (IF): IF : 1:50-1:500
• Durchflusszytometrie (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL555-80297 bindet in IF, FC (Intra) CD45 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD45 fusion protein Ag13712
• Vollständiger Name: protein tyrosine phosphatase, receptor type, C
• Berechnetes Molekulargewicht: 1304 aa, 147 kDa
• Beobachtetes Molekulargewicht: 220-240 kDa
• GenBank-Zugangsnummer: BC014239
• Gene symbol: PTPRC
• Gene ID (NCBI): 5788
• Konjugation: CoraLite®555 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 557 nm / 570 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: BS mit 50% Glyzerin, 0,05% Proclin300, 0,5% BSA, pH 7,3.
• Lagerungsbedingungen: Bei -20°C lagern. Vor Licht schützen. Nach dem Versand ein Jahr stabil. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD45, also known as leukocyte common antigen (LCA), B220, and T200, is a 180-240 kD single chain type I membrane glycoprotein. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 is one of the best negative selection markers for characterizing and/or isolating human mesenchymal stem cells from bone marrow and other sources. Along with the positive selection markers (such as CD29, CD44, CD90, CD105 and CD166), negative selection markers (such as CD34 and CD45) are used for MSC identification.

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sonja (Verified Customer) (01-27-2023)

The antibody is perfect. No background and very strong specific signal!

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: FFPE Tonsil

CL555 CD45 Antibody Immunofluorescence validation (1:50 dilution) in FFPE Tonsil (Cat no:CL555-80297)