CD45 Rekombinanter Antikörper

CD45 Rekombinant Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

240356G10

Kat-Nr. : 83396-5-PBS

Synonyme

CD45/PTPRC, Ptprc, 240356G10, EC:3.1.3.48, LCA

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  WB analysis using 83396-5-RR (same clone as 83396-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83396-5-RR (CD45 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83396-5-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 83396-5-RR (same clone as 83396-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83396-5-RR (CD45 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83396-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse colon using 83396-5-RR (same clone as 83396-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 83396-5-RR (CD45 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83396-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 83396-5-RR (same clone as 83396-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83396-5-RR (CD45 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83396-5-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse spleen using 83396-5-RR (same clone as 83396-5-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83396-5-RR (CD45 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83396-5-PBS in a different storage buffer formulation.

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  IF Staining of RAW 264.7 using 83396-5-RR (same clone as 83396-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD45/PTPRC antibody (83396-5-RR, Clone: 240356G10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83396-5-PBS in a different storage buffer formulation.

• 

  IF Staining of RAW 264.7 using 83396-5-RR (same clone as 83396-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed RAW 264.7 cells using CD45/PTPRC antibody (83396-5-RR, Clone: 240356G10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83396-5-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 83396-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83396-5-RR against Mouse CD45 were performed. The affinity constant is 0.415 nM.

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Geprüfte Anwendungen

Produktinformation

83396-5-PBS bindet in WB, IHC, IF/ICC, ELISA CD45 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: CD45 fusion protein Eg0845
• Vollständiger Name: protein tyrosine phosphatase, receptor type, C
• Berechnetes Molekulargewicht: 145 kDa
• Beobachtetes Molekulargewicht: 220 kDa
• GenBank-Zugangsnummer: NM_001111316.2
• Gene symbol: CD45
• Gene ID (NCBI): 19264
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097).