CD45R (B220) Monoklonaler Antikörper

CD45R (B220) Monoklonal Antikörper für IF/ICC, FC

Wirt / Isotyp

Ratte / IgG2a, kappa

Getestete Reaktivität

Maus

Anwendung

IF/ICC, FC

Konjugation

CoraLite® Plus 647 Fluorescent Dye

CloneNo.

RA3-6B2

Kat-Nr. : CL647-65139

Synonyme

B220, CD45R, Cd45, Ptprc, EC:3.1.3.48

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  IF Staining of mouse splenocytes using CL647-65139

  Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 647-conjugated CD45R (B220) antibody (CL647-65139, Clone: RA3-6B2, red) at dilution of 1:400, and CoraLite®488-conjugated CD3 antibody (CL488-65077, Clone: 17A2, green).

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  FC experiment of C57BL/c mouse splenocytes using CL647-65139

  1x10^6 C57BL/c mouse splenocytes were surface stained with 0.25 ug CoraLite® Plus 647 Anti-Mouse CD45R (B220) (CL647-65139, Clone:RA3-6B2) or 0.25 ug CoraLite® Plus 647 Rat IgG2a Isotype Control (2A3) (CL647-65209, Clone: 2A3). Cells were co-stained with PE Anti-Mouse CD3 (17A2) (PE-65077, Clone: 17A2). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: Maus-Splenozyten
• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

CL647-65139 bindet in IF/ICC, FC CD45R (B220) und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Murines Abelson-Leukämie-virusinduzierte Vorläufer-B-Tumorzellen
• Vollständiger Name: protein tyrosine phosphatase, receptor type, C
• GenBank-Zugangsnummer: BC028512
• Gene symbol: CD45
• Gene ID (NCBI): 19264
• Konjugation: CoraLite® Plus 647 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 654 nm / 674 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45R (B220) is an isoform of CD45, expressed on murine B-lineage cells (except for plasma cells) and on a small sub-population of T cells (PMID: 6975314; 15712182). It is commonly used as a pan B-cell marker in mice. CD45R is also expressed on activated T cells and NK cell subsets (PMID: 7514808; 17923504). The RA3-6B2 clone reacts with human and mouse CD45R.