CD45R (B220) Monoklonaler Antikörper

CD45R (B220) Monoklonal Antikörper für IF/ICC, FC

Wirt / Isotyp

Ratte / IgG2a, kappa

Getestete Reaktivität

Maus

Anwendung

IF/ICC, FC

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

RA3-6B2

Kat-Nr. : 65139-1-Ig

Synonyme

B220, CD45R, Cd45, Ptprc, EC:3.1.3.48

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  IF Staining of mouse splenocytes using 65139-1-Ig

  Immunofluorescent analysis of mouse splenocytes using CD45R (B220) antibody (65139-1-Ig, Clone: RA3-6B2 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rat IgG(H+L).

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  FC experiment of C57BL/c mouse splenocytes using 65139-1-Ig

  1X10^6 C57BL/c mouse splenocytes were surface co-stained with APC Anti-Mouse CD3 and 0.5 ug Anti-Mouse CD45R (B220) (65139-1-Ig, Clone: RA3-6B2) or 0.5 ug Isotype Control. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IF/ICC: Maus-Splenozyten
• Erfolgreiche Detektion in FC: Maus-Splenozyten

Empfohlene Verdünnung

• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:250-1:1000

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• IF: See 1 publications below

Produktinformation

65139-1-Ig bindet in IF/ICC, FC CD45R (B220) und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• In Publikationen genannte Reaktivität: Maus
• Wirt / Isotyp: Ratte / IgG2a, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Murines Abelson-Leukämie-virusinduzierte Vorläufer-B-Tumorzellen
• Vollständiger Name: protein tyrosine phosphatase, receptor type, C
• GenBank-Zugangsnummer: BC028512
• Gene symbol: CD45
• Gene ID (NCBI): 19264
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signalling (PMID: 9429890; 16378097). CD45R (B220) is an isoform of CD45, expressed on murine B-lineage cells (except for plasma cells) and on a small sub-population of T cells (PMID: 6975314; 15712182). It is commonly used as a pan B-cell marker in mice. CD45R is also expressed on activated T cells and NK cell subsets (PMID: 7514808; 17923504). The RA3-6B2 clone reacts with human and mouse CD45R.

Publikationen

Species	Application	Title
mouse	IF	Thorac Cancer; Experimental model of micronodular thymic neoplasm with lymphoid stroma; Authors - Riko Niwa