CD45RA Rekombinanter Antikörper

CD45RA Rekombinant Antikörper für FC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Ratte

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

OX-33

Kat-Nr. : 65578-1-MR

Synonyme

CD45, Lca, Ptprc, L CA, OX-33

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  FC experiment of rat splenocytes using 65578-1-MR

  1x10^6 rat splenocytes were surface stained with 0.25 ug Anti-Rat CD45RA Mouse IgG2a Recombinant Antibody (65578-1-MR, Clone: OX-33) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and FITC anti-Mouse IgG2a Antibody. Cells were then stained with APC Anti-Rat CD4. Cells were not fixed.

• 

  FC experiment of rat splenocytes using 65578-1-MR

  1x10^6 rat splenocytes were surface stained with 0.25 ug Anti-Rat CD45RA Mouse IgG2a Recombinant Antibody (65578-1-MR, Clone:OX-33) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and FITC anti-Mouse IgG2a Antibody. Cells were not fixed.

• 

  FC experiment of rat splenocytes using 65578-1-MR

  1x10^6 rat splenocytes were surface stained with 0.25 ug Anti-Rat CD45RA Mouse IgG2a Recombinant Antibody (65578-1-MR, Clone: OX-33) and FITC anti-Mouse IgG2a Antibody (left), or 0.25 ug Anti-Rat CD45RA (65234-1-Ig, Clone: OX-33) and FITC anti-Mouse IgG1 Antibody (right). Cells were then stained with APC Anti-Rat CD4. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Ratten-Splenozyten

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

65578-1-MR bindet in FC CD45RA und zeigt Reaktivität mit Ratten

• Getestete Reaktivität: Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Leukocyte common antigen purified from rat splenocytes
• Vollständiger Name: protein tyrosine phosphatase, receptor type, C
• Berechnetes Molekulargewicht: 143 kDa
• GenBank-Zugangsnummer: XM_006249910
• Gene symbol: CD45
• Gene ID (NCBI): 24699
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signaling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).