CD45RA Rekombinanter Antikörper

CD45RA Rekombinant Antikörper für FC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Ratte

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

OX-33

Kat-Nr. : 65578-1-PBS

Synonyme

CD45, Lca, Ptprc, L CA, OX-33

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  FC experiment of rat splenocytes using 65578-1-MR (same clone as 65578-1-PBS)

  1x10^6 rat splenocytes were surface stained with 0.25 ug Anti-Rat CD45RA Mouse IgG2a Recombinant Antibody (65578-1-MR, Clone: OX-33) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) and FITC anti-Mouse IgG2a Antibody. Cells were then stained with APC Anti-Rat CD4. Cells were not fixed. This data was developed using the same antibody clone with 65578-1-PBS in a different storage buffer formulation.

• 

  FC experiment of rat splenocytes using 65578-1-MR (same clone as 65578-1-PBS)

  1x10^6 rat splenocytes were surface stained with 0.25 ug Anti-Rat CD45RA Mouse IgG2a Recombinant Antibody (65578-1-MR, Clone:OX-33) (red) or Mouse IgG2a Isotype Control (65208-1-Ig, Clone: C1.18.4) (blue) and FITC anti-Mouse IgG2a Antibody. Cells were not fixed. This data was developed using the same antibody clone with 65578-1-PBS in a different storage buffer formulation.

• 

  FC experiment of rat splenocytes using 65578-1-MR (same clone as 65578-1-PBS)

  1x10^6 rat splenocytes were surface stained with 0.25 ug Anti-Rat CD45RA Mouse IgG2a Recombinant Antibody (65578-1-MR, Clone: OX-33) and FITC anti-Mouse IgG2a Antibody (left), or 0.25 ug Anti-Rat CD45RA (65234-1-Ig, Clone: OX-33) and FITC anti-Mouse IgG1 Antibody (right). Cells were then stained with APC Anti-Rat CD4. Cells were not fixed. This data was developed using the same antibody clone with 65578-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

65578-1-PBS bindet in FC CD45RA und zeigt Reaktivität mit Ratten

• Getestete Reaktivität: Ratte
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Leukocyte common antigen purified from rat splenocytes
• Vollständiger Name: protein tyrosine phosphatase, receptor type, C
• Berechnetes Molekulargewicht: 143 kDa
• GenBank-Zugangsnummer: XM_006249910
• Gene symbol: CD45
• Gene ID (NCBI): 24699
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

CD45, also known as protein tyrosine phosphatase, receptor type C, is a type I transmembrane protein expressed on the surface of all haematopoietic cells with the exception of erythrocytes and platelets (PMID: 3489673; 28615666). CD45 is a pan-haematopoietic cell marker and has been shown to be essential for T- and B-cell activation and signaling (PMID: 9429890; 16378097). CD45 exists as multiple isoforms due to alternative splicing of three exons (4, 5, and 6, designated A, B, and C) in the extracellular domain (PMID: 12414720). CD45RA is expressed on naïve T cells, B cells, and monocytes (PMID: 1830500; 14687231).